Product nameAnti-PFKM antibody (HRP)
See all PFKM primary antibodies
DescriptionGoat polyclonal to PFKM (HRP)
Tested applicationsSuitable for: WB, ELISA, Immunomicroscopy, Dot blotmore details
Species reactivityReacts with: Rabbit
Full length native protein (purified) corresponding to Rabbit PFKM.
This product was previously labelled as Fructose 6 Phosphate Kinase
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesPurified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab34605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/1,000 - 1/3,500. Assayed against 1.0 ug of Fructose-6-Phosphate Kinase
(from Rabbit Muscle) in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 85 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Involvement in diseaseDefects in PFKM are the cause of glycogen storage disease type 7 (GSD7) [MIM:232800]; also known as Tarui disease. GSD7 is an autosomal recessive disorder characterized by exercise intolerance with associated nausea and vomiting. Short bursts of intense activity are particularly difficult. Severe muscle cramps and myoglobinuria develop after vigorous exercise. Most patients obtain a "second wind" when the onset of exercise is followed by a brief rest period. In time patients adjust their activity level and are well compensated.
Sequence similaritiesBelongs to the phosphofructokinase family. Two domains subfamily.
- Information by UniProt
- 6 Phosphofructokinase Muscle Type antibody
- 6-phosphofructokinase antibody
- 6-phosphofructokinase muscle type antibody
ab34605 has not yet been referenced specifically in any publications.