Key features and details
- Rabbit polyclonal to PFKP
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PFKP antibody
See all PFKP primary antibodies
DescriptionRabbit polyclonal to PFKP
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
- HeLa, 293T, Jurkat and NIH 3T3 whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab186132 was affinity purified using an epitope specific to PFKP immobilized on solid support.
Our Abpromise guarantee covers the use of ab186132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 86 kDa.|
PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Sequence similaritiesBelongs to the phosphofructokinase family. Two domains subfamily.
- Information by UniProt
- 1200015H23Rik antibody
- 6 phosphofructokinase type C antibody
- 6 phosphofructokinase, platelet type antibody
All lanes : Anti-PFKP antibody (ab186132) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 3 minutes
Detection of PFKP in immunoprecipitates of 293T whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) using ab186132 at 6 µg/reaction for IP. For WB detection, ab186132 was used at 1µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab186132 has been referenced in 1 publication.
- Wang H et al. The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival. Nature 546:426-430 (2017). PubMed: 28607489