Anti-PFKP antibody (ab233109)
Key features and details
- Rabbit polyclonal to PFKP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PFKP antibody
See all PFKP primary antibodies -
Description
Rabbit polyclonal to PFKP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Orangutan -
Immunogen
Recombinant fragment (His-tag) corresponding to Human PFKP aa 550-800. Expressed in E.coli.
Database link: Q01813 -
Positive control
- WB: Recombinant human PFKP protein; A549 and Jurkat whole cell lysate (ab7899); Pig heart and skeletal muscle lysates. IHC-P: Human lung cancer, liver, brain, breast cancer, prostate cancer and stomach tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 86 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 86 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. -
Sequence similarities
Belongs to the phosphofructokinase family. Two domains subfamily. - Information by UniProt
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Database links
- Entrez Gene: 5214 Human
- Entrez Gene: 56421 Mouse
- Entrez Gene: 100173619 Orangutan
- Entrez Gene: 60416 Rat
- Omim: 171840 Human
- SwissProt: Q01813 Human
- SwissProt: Q9WUA3 Mouse
- SwissProt: Q5R636 Orangutan
see all -
Alternative names
- 1200015H23Rik antibody
- 6 phosphofructokinase type C antibody
- 6 phosphofructokinase, platelet type antibody
see all
Images
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Anti-PFKP antibody (ab233109) at 5 µg/ml + Recombinant human PFKP protein
Predicted band size: 86 kDa -
All lanes : Anti-PFKP antibody (ab233109) at 3 µg/ml
Lane 1 : A549 (human lung carcinoma cell line) cell lysate
Lane 2 : Pig heart lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : Pig skeletal muscle lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 86 kDa -
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human brain tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for PFKP using ab233109 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233109 has not yet been referenced specifically in any publications.