Anti-PFKP antibody - C-terminal (ab167083)
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- References
- Protocols
Overview
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Product nameAnti-PFKP antibody - C-terminal
See all PFKP primary antibodies -
DescriptionRabbit polyclonal to PFKP - C-terminal
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide conjugated to KLH, derived from the C terminal region of Human PFKP.
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Positive control
- T47D cell line lysate; Human kidney tubules; Fibroblasts.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein G purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab167083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/500. Predicted molecular weight: 86 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. |
Target
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PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
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Sequence similaritiesBelongs to the phosphofructokinase family. Two domains subfamily.
- Information by UniProt
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Database links
- Entrez Gene: 5214 Human
- Omim: 171840 Human
- SwissProt: Q01813 Human
- Unigene: 26010 Human
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Alternative names
- 1200015H23Rik antibody
- 6 phosphofructokinase type C antibody
- 6 phosphofructokinase, platelet type antibody
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Images
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Western blot - Anti-PFKP antibody - C-terminal (ab167083)Anti-PFKP antibody - C-terminal (ab167083) at 1/100 dilution + T47D cell line lysate at 35 µg
Predicted band size: 86 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKP antibody - C-terminal (ab167083)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tubules labeling PFKP with ab167083 at 5 µg/ml. -
Western blot - Anti-PFKP antibody - C-terminal (ab167083)Fibroblasts were cultured for 2 d in DMEM supplemented with 10% TH-depleted bovine serum. They were then treated with 2 nM T3 in the absence or presence of either LY294002 (LY, 50 M) or PD98059 (PD, 50 M), added 1 h before the addition of T3. Cells were harvested 24 h after T3 treatment. Whole-cell lysates, obtained from two separately treated culture dishes for each treatment, were subjected to WB with ab167083 at 1/100 dilution.
Datasheets and documents
References
ab167083 has not yet been referenced specifically in any publications.
