Recombinant
RabMAb

Recombinant Anti-PFKP antibody [EPR17314] (ab204131)

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Overview

  • Product name

    Anti-PFKP antibody [EPR17314]
    See all PFKP primary antibodies

  • Description

    Rabbit monoclonal [EPR17314] to PFKP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human PFKP aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: Q01813

  • Positive control

    • WB: MCF7, HeLa, Raji, Jurkat and NIH/3T3 whole cell lsates; Human fetal brain and fetal kidney lysates; Mouse and rat kidney and spleen lysates. ICC/IF: HeLa and MCF7 cells. Flow Cyt: Jurkat and MCF7 cells. IP: MCF7 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab204131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
ICC/IF 1/250.
IP 1/80.
Flow Cyt 1/250.

Target

  • Pathway

    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

  • Sequence similarities

    Belongs to the phosphofructokinase family. Two domains subfamily.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 1200015H23Rik antibody
    • 6 phosphofructokinase type C antibody
    • 6 phosphofructokinase, platelet type antibody
    • 6-phosphofructokinase antibody
    • 6-phosphofructokinase type C antibody
    • 9330125N24Rik antibody
    • FLJ40226 antibody
    • K6PP antibody
    • K6PP_HUMAN antibody
    • MGC105718 antibody
    • PFK C antibody
    • PFK, fibroblast type antibody
    • PFK-C antibody
    • PFKF antibody
    • PFKP antibody
    • Phosphofructo 1 kinase isozyme C antibody
    • Phosphofructo-1-kinase isozyme C antibody
    • Phosphofructokinase 1 antibody
    • Phosphofructokinase platelet antibody
    • Phosphohexokinase antibody
    • platelet type antibody
    see all

Images

  • Western blot - Anti-PFKP antibody [EPR17314] (ab204131)
    Western blot - Anti-PFKP antibody [EPR17314] (ab204131)
    All lanes : Anti-PFKP antibody [EPR17314] (ab204131) at 1/20000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 86 kDa
    Observed band size: 86 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PFKP antibody [EPR17314] (ab204131)
    Western blot - Anti-PFKP antibody [EPR17314] (ab204131)
    All lanes : Anti-PFKP antibody [EPR17314] (ab204131) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 86 kDa
    Observed band size: 86 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PFKP antibody [EPR17314] (ab204131)
    Western blot - Anti-PFKP antibody [EPR17314] (ab204131)
    All lanes : Anti-PFKP antibody [EPR17314] (ab204131) at 1/2000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 86 kDa
    Observed band size: 86 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKP antibody [EPR17314] (ab204131)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKP antibody [EPR17314] (ab204131)

    Immunofluorescent analysis of 100% methanl-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling PFKP with ab204131 at 1/250 dilution, followed by Goat Anti-Rabbit IgG  H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with  Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab204131 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2:  Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody  at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKP antibody [EPR17314] (ab204131)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKP antibody [EPR17314] (ab204131)

    Immunofluorescent analysis of 100% methanl-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling PFKP with ab204131 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab204131 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-PFKP antibody [EPR17314] (ab204131)
    Flow Cytometry - Anti-PFKP antibody [EPR17314] (ab204131)

    Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling PFKP with ab204131 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-PFKP antibody [EPR17314] (ab204131)
    Flow Cytometry - Anti-PFKP antibody [EPR17314] (ab204131)

    Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling PFKP with ab204131 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-PFKP antibody [EPR17314] (ab204131)
    Immunoprecipitation - Anti-PFKP antibody [EPR17314] (ab204131)

    PFKP was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab204131 at 1/80 dilution.

    Western blot was performed from the immunoprecipitate using ab204131 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: MCF7 whole cell lysate 10ug (Input).

    Lane 2: ab204131 IP in MCF7 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab204131 in MCF7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

References

This product has been referenced in:

  • Kanai S  et al. Phosphofructokinase-1 subunit composition and activity in the skeletal muscle, liver, and brain of dogs. J Vet Med Sci 81:712-716 (2019). Read more (PubMed: 30918224) »
  • Zhou K  et al. VDAC2 interacts with PFKP to regulate glucose metabolism and phenotypic reprogramming of glioma stem cells. Cell Death Dis 9:988 (2018). Read more (PubMed: 30250190) »
See all 2 Publications for this product

Publishing research using ab204131? Please let us know so that we can cite the reference in this datasheet.

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