RecombinantRabMAb

Recombinant Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)

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Immunocytochemistry/ Immunofluorescence - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)
  • Immunocytochemistry/ Immunofluorescence - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)
  • Flow Cytometry - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)
  • Flow Cytometry - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)
  • Immunoprecipitation - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)
  • Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17314] to PFKP - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PFKP antibody [EPR17314] - BSA and Azide free
    See all PFKP primary antibodies

  • Description

    Rabbit monoclonal [EPR17314] to PFKP - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 79%  identity (88% positives) with PFKM. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa and MCF7 cells. Flow Cyt: Jurkat and MCF7 cells. IP: MCF7 whole cell lysate.

  • General notes

    Ab242413 is the carrier-free version of ab204131. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab242413 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab242413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IP
Human
Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
Notes
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).

Target

  • Pathway

    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

  • Sequence similarities

    Belongs to the phosphofructokinase family. Two domains subfamily.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 1200015H23Rik antibody
    • 6 phosphofructokinase type C antibody
    • 6 phosphofructokinase, platelet type antibody
    • 6-phosphofructokinase antibody
    • 6-phosphofructokinase type C antibody
    • 9330125N24Rik antibody
    • FLJ40226 antibody
    • K6PP antibody
    • K6PP_HUMAN antibody
    • MGC105718 antibody
    • PFK C antibody
    • PFK, fibroblast type antibody
    • PFK-C antibody
    • PFKF antibody
    • PFKP antibody
    • Phosphofructo 1 kinase isozyme C antibody
    • Phosphofructo-1-kinase isozyme C antibody
    • Phosphofructokinase 1 antibody
    • Phosphofructokinase platelet antibody
    • Phosphohexokinase antibody
    • platelet type antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)

    Immunofluorescent analysis of 100% methanl-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling PFKP with ab204131 at 1/250 dilution, followed by Goat Anti-Rabbit IgG  H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with  Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab204131 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2:  Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody  at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab204131)
  • Immunocytochemistry/ Immunofluorescence - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)
    Immunocytochemistry/ Immunofluorescence - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)

    Immunofluorescent analysis of 100% methanl-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling PFKP with ab204131 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab204131 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab204131)
  • Flow Cytometry - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)
    Flow Cytometry - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)

    Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling PFKP with ab204131 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab204131)
  • Flow Cytometry - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)
    Flow Cytometry - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)

    Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling PFKP with ab204131 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab204131)
  • Immunoprecipitation - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)
    Immunoprecipitation - Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)

    PFKP was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab204131 at 1/80 dilution.

    Western blot was performed from the immunoprecipitate using ab204131 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: MCF7 whole cell lysate 10ug (Input).

    Lane 2: ab204131 IP in MCF7 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab204131 in MCF7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab204131)
  • Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)
    Anti-PFKP antibody [EPR17314] - BSA and Azide free (ab242413)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab242413? Please let us know so that we can cite the reference in this datasheet.

ab242413 has not yet been referenced specifically in any publications.

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