Overview

  • Product name

    Anti-PFKP antibody [OTI1D6]
    See all PFKP primary antibodies
  • Description

    Mouse monoclonal [OTI1D6] to PFKP
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length Human PFKP produced in HEK293T cells (NP_002618).

  • Positive control

    • HEK293T cell lysate transfected with pCMV6-ENTRY PFKP; SVT2, A549, Jurkat, PC12 and MCF7 cell extracts; Human colon adenocarcinoma, kidney carcinoma, lung carcinoma, ovary adenocarcinoma, endometrium, endometrium adenocarcinoma, bladder carcinoma and tonsil tissues; COS7 cells transiently transfected with pCMV6-ENTRY PFKP; Jurkat cells.
  • General notes

    The clone number has been updated from 1D6 to OTI1D6, both clone numbers name the same clone.

    Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab119796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 86 kDa.
IHC-P 1/150.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100.

Target

  • Pathway

    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
  • Sequence similarities

    Belongs to the phosphofructokinase family. Two domains subfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1200015H23Rik antibody
    • 6 phosphofructokinase type C antibody
    • 6 phosphofructokinase, platelet type antibody
    • 6-phosphofructokinase antibody
    • 6-phosphofructokinase type C antibody
    • 9330125N24Rik antibody
    • FLJ40226 antibody
    • K6PP antibody
    • K6PP_HUMAN antibody
    • MGC105718 antibody
    • PFK C antibody
    • PFK, fibroblast type antibody
    • PFK-C antibody
    • PFKF antibody
    • PFKP antibody
    • Phosphofructo 1 kinase isozyme C antibody
    • Phosphofructo-1-kinase isozyme C antibody
    • Phosphofructokinase 1 antibody
    • Phosphofructokinase platelet antibody
    • Phosphohexokinase antibody
    • platelet type antibody
    see all

Images

  • All lanes : Anti-PFKP antibody [OTI1D6] (ab119796) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 86 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200673)
  • All lanes : Anti-PFKP antibody [OTI1D6] (ab119796) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PFKP cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 86 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200673)
  • ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Colon tissue by Immunohistochemistry.
  • ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Kidney carcinoma tissue by Immunohistochemistry.
  • ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Lung carcinoma tissue by Immunohistochemistry.
  • ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Ovary adenocarcinoma tissue by Immunohistochemistry.
  • ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Endometrium tissue by Immunohistochemistry.
  • ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Bladder carcinoma tissue by Immunohistochemistry.
  • ab119796 at 1/150 dilution staining PFKP in Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
  • ab119796 at 1/100 dilution staining PFKP in COS7 cells transiently transfected with pCMV6-ENTRY PFKP by Immunofluorescence.
  • ab119796 at 1/100 dilution staining PFKP in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

References

This product has been referenced in:

  • Stancic A  et al. A lesson from the oxidative metabolism of hibernator heart: Possible strategy for cardioprotection. Comp Biochem Physiol B Biochem Mol Biol 219-220:1-9 (2018). WB . Read more (PubMed: 29501789) »
  • Yang XY  et al. Energy Metabolism Disorder as a Contributing Factor of Rheumatoid Arthritis: A Comparative Proteomic and Metabolomic Study. PLoS One 10:e0132695 (2015). Read more (PubMed: 26147000) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
I am sending you our WB questionnaire as I would like to focus on one application first. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
I look forward to receiving your reply.
Order Details Antibody code: ab119796 Anti-PFKP antibody [1D6]
Problem Choose: Non-specific band Multiple bands No signal or weak signal High background
Lot number
Purchase order number or preferably Abcam order number:
General Information Antibody storage conditions (temperature/reconstitution etc)
Description of the problem (high background, wrong band size, more bands, no band etc.)
Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)
Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)
Amount of protein loaded
Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)
Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)
Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Detection method (ECL, ECLPlus etc.)
Positive and negative controls used (please specify)
Optimization attempts (problem solving) How many times have you tried the Western?
Have you run a "No Primary" control? Yes No
Do you obtain the same results every time? Yes No e.g. are the background bands always in the same place?
What steps have you altered?
Additional Notes:
Image: We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asses the results.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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