Anti-PFTK1 antibody - N-terminal (ab175489)

Overview

  • Product name
    Anti-PFTK1 antibody - N-terminal
    See all PFTK1 primary antibodies
  • Description
    Rabbit polyclonal to PFTK1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human PFTK1 aa 74-104 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O94921

  • Positive control
    • Mouse cerebellum tissue lysate; Human brain tissue; HepG2 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab175489 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 53 kDa.

Target

  • Function
    Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport.
  • Tissue specificity
    Highly expressed in brain, pancreas, kidney, heart, testis and ovary. Also detected at lower levels in other tissues except in spleen and thymus where expression is barely detected.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cell membrane. Cytoplasm. Nucleus. Recruited to the cell membrane by CCNY.
  • Information by UniProt
  • Database links
  • Alternative names
    • cdk14 antibody
    • CDK14_HUMAN antibody
    • Cell division protein kinase 14 antibody
    • Cyclin-dependent kinase 14 antibody
    • hPFTAIRE1 antibody
    • KIAA0834 antibody
    • mKIAA0834 antibody
    • PFTAIRE protein kinase 1 antibody
    • PFTAIRE1 antibody
    • PFTK1 antibody
    • Serine/threonine protein kinase PFTAIRE 1 antibody
    • Serine/threonine-protein kinase PFTAIRE-1 antibody
    see all

Images

  • Anti-PFTK1 antibody - N-terminal (ab175489) at 1/1000 dilution + Mouse cerebellum tissue lysate at 35 µg

    Predicted band size: 53 kDa

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain tissue labeling PFTK1 with ab175489 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of HepG2 cells labeling PFTK1 with ab175489 antibody at 1/10 dilution (right histogram), compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175489 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175489.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up