Key features and details
- Mouse monoclonal [7G3] to PGA3
- Suitable for: IHC-P, ELISA, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-PGA3 antibody [7G3]
DescriptionMouse monoclonal [7G3] to PGA3
Tested applicationsSuitable for: IHC-P, ELISA, Sandwich ELISAmore details
Species reactivityReacts with: Human
Full length native protein (purified)
This product was changed from ascites to tissue culture supernatant on 11th September, 2017. Lot numbers including and higher than GR3182440-1, GR3182440-2, and GR3182440-3 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.04% PBS, 0.87% Sodium chloride
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab50123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ELISA||Use a concentration of 1 - 10 µg/ml.|
|Sandwich ELISA||Use at an assay dependent concentration.|
RelevancePepsinogen I is proenzyme that is cleaved to form pepsin both in an autocatalytic fashion and by pepsin itself.
- Pepsinogen 1 antibody
- Pepsinogen A antibody
- Pepsinogen antibody
IHC image of ab50123 staining in human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50123, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab50123 has not yet been referenced specifically in any publications.