Key features and details
- Goat polyclonal to PGAM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PGAM1 antibody
See all PGAM1 primary antibodies
DescriptionGoat polyclonal to PGAM1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Monkey
- Human liver and brain lysates.
General notesGenBank Accession Number – NP_002620.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab2220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 28 kDa.
1 hour primary incubation is recommended for this product.
FunctionInterconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 126.96.36.199 (synthase) and EC 188.8.131.52 (phosphatase), but with a reduced activity.
Tissue specificityIn mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues.
Sequence similaritiesBelongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
- Information by UniProt
- BPG dependent PGAM 1 antibody
- BPG dependent PGAM1 antibody
- BPG-dependent PGAM 1 antibody
Anti-PGAM1 antibody (ab2220) at 0.01 µg/ml + Human liver lysate at 35 µg
Developed using the ECL technique.
Primary incubated for 1 hour
ab2220 staining (0.5
µg/ml) of Human Liver lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ab2220 has been referenced in 6 publications.
- Okuda J et al. Persistent overexpression of phosphoglycerate mutase, a glycolytic enzyme, modifies energy metabolism and reduces stress resistance of heart in mice. PLoS One 8:e72173 (2013). WB ; Mouse . PubMed: 23951293
- Hallows WC et al. Regulation of Glycolytic Enzyme Phosphoglycerate Mutase-1 by Sirt1 Protein-mediated Deacetylation. J Biol Chem 287:3850-8 (2012). WB ; Human . PubMed: 22157007
- Lei Y et al. Proteomics Identification of ITGB3 as a Key Regulator in Reactive Oxygen Species-induced Migration and Invasion of Colorectal Cancer Cells. Mol Cell Proteomics 10:M110.005397 (2011). WB, IHC-P ; Human . PubMed: 21622897
- Klein-Scory S et al. Immunoscreening of the extracellular proteome of colorectal cancer cells. BMC Cancer 10:70 (2010). WB ; Human . PubMed: 20184735
- Evans MJ et al. Mechanistic and structural requirements for active site labeling of phosphoglycerate mutase by spiroepoxides. Mol Biosyst 3:495-506 (2007). PubMed: 17579775
- Evans MJ et al. Target discovery in small-molecule cell-based screens by in situ proteome reactivity profiling. Nat Biotechnol 23:1303-7 (2005). PubMed: 16200062