Product nameAnti-PGAM1 antibody
See all PGAM1 primary antibodies
DescriptionRabbit polyclonal to PGAM1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human adrenal gland tissue. ICC/IF: PC-3 cells. WB: RT4 and U-251 MG cell lysate; Human liver and tonsil tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab247037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.4 µg/ml.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
FunctionInterconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 126.96.36.199 (synthase) and EC 188.8.131.52 (phosphatase), but with a reduced activity.
Tissue specificityIn mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues.
Sequence similaritiesBelongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
- Information by UniProt
- BPG dependent PGAM 1 antibody
- BPG dependent PGAM1 antibody
- BPG-dependent PGAM 1 antibody
PFA fixed, Triton X-100 permeabilized PC-3 (Human prostate adenocarcinoma cell line) cells labeling PGAM1 using ab247037 at 4 µg/ml (green) in ICC/IF.
Paraffin-embedded human adrenal gland tissue stained for PGAM1 using ab247037 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-PGAM1 antibody (ab247037) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
ab247037 has not yet been referenced specifically in any publications.