Overview

  • Product name

    Anti-PGAM1 antibody [EPR8042]
    See all PGAM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8042] to PGAM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PGAM1 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat, Human fetal muscle, A431, and Raji cell lysates; permeabilized Raji cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.
    • Tissue specificity

      In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues.
    • Sequence similarities

      Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
    • Information by UniProt
    • Database links

    • Alternative names

      • BPG dependent PGAM 1 antibody
      • BPG dependent PGAM1 antibody
      • BPG-dependent PGAM 1 antibody
      • OTTHUMP00000059414 antibody
      • PGAM 1 antibody
      • PGAM A antibody
      • PGAM B antibody
      • PGAM-B antibody
      • PGAM1 antibody
      • PGAM1_HUMAN antibody
      • PGAMA antibody
      • PGAMB antibody
      • Phosphoglycerate mutase 1 antibody
      • Phosphoglycerate mutase 1 brain antibody
      • Phosphoglycerate mutase A antibody
      • Phosphoglycerate mutase A nonmuscle form antibody
      • Phosphoglycerate mutase isozyme B antibody
      see all

    Images

    • All lanes : Anti-PGAM1 antibody [EPR8042] (ab129191) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : Human fetal muscle cell lysates
      Lane 3 : A431 cell lysates
      Lane 4 : Raji cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 29 kDa

    • ab129191 at 1/100 dilution staining PGAM1 in permeabilized Raji cells by Flow cytometry or a rabbit IgG negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Zhao Y & Zhang S PGAM1 knockdown is associated with busulfan-induced hypospermatogenesis and spermatogenic cell apoptosis. Mol Med Rep 19:2497-2502 (2019). Read more (PubMed: 30720109) »
    • Zhang D  et al. Phosphoglycerate Mutase 1 Predicts the Poor Prognosis of Oral Squamous Cell Carcinoma and is Associated with Cell Migration. J Cancer 8:1943-1951 (2017). Read more (PubMed: 28819393) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab129191.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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