Overview

  • Product name

  • Description

    Rabbit polyclonal to PGAM2
  • Host species

    Rabbit
  • Specificity

    The immunogen sequence shows 81% homology with PGAM1, so cross-reactivity with PGAM1 cannot be excluded. However, cross-reactivity has not been tested.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-203 of Human PGAM2 (NP_000281)

  • Positive control

    • H1299 or HeLa lysates A549 cells (IF)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab97800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.
  • Tissue specificity

    In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues.
  • Involvement in disease

    Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10) [MIM:261670]. A metabolic disorder characterized by myoglobinuria, increased serum creatine kinase levels, decreased phosphoglycerate mutase activity, myalgia, muscle pain, muscle cramps and excercise intolerance.
  • Sequence similarities

    Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • BPG dependent PGAM 2 antibody
    • BPG-dependent PGAM 2 antibody
    • GSD10 antibody
    • MGC88743 antibody
    • Muscle specific phosphoglycerate mutase antibody
    • Muscle-specific phosphoglycerate mutase antibody
    • OTTHUMP00000207787 antibody
    • PGAM 2 antibody
    • PGAM M antibody
    • PGAM-M antibody
    • Pgam2 antibody
    • PGAM2_HUMAN antibody
    • PGAMM antibody
    • phosphoglycerate mutase 2 (muscle) antibody
    • Phosphoglycerate mutase 2 antibody
    • Phosphoglycerate mutase isozyme M antibody
    see all

Images

  • All lanes : Anti-PGAM2 antibody (ab97800) at 1/10000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 29 kDa



    12% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab97800 at 1/200 dilution. Lower image was also merged with DNA probe.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry for the unclear information on the datashet. As requested, I have issued a free of charge replacement of ab119185.

This is coming from our lab and you can expected it on March 20th or 21st. To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question
Answer

Thank you for your inquiry.


The lab confirmed that they have not done specificity tests for ab97800 for cross-reactivity with PGAM1. They BLASTed the immunogen sequence with PGAM1, which shows 81% homology, so they could not exclude cross-reactivity with PGAM1, but again this has not been verified.


I understand that you purchased this product unaware of this potential cross-reactivity issue. If you would like, I would be happy to send you a vial of a PGAM1 protein (ab100885).


https://www.abcam.com/PGAM1-protein-ab100885.html


Afterward, If the antibody detects the PGAM1 protein, we would then refund your antibody purchase and rename the product.


Would you be able to test this and send us the results? Let me know how you would like to proceed.

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