Key features and details
- Rabbit polyclonal to PGAM2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PGAM2 antibody
See all PGAM2 primary antibodies
DescriptionRabbit polyclonal to PGAM2
SpecificityThe immunogen sequence shows 81% homology with PGAM1, so cross-reactivity with PGAM1 cannot be excluded. However, cross-reactivity has not been tested.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Recombinant fragment, corresponding to a region within amino acids 1-203 of Human PGAM2 (NP_000281)
- H1299 or HeLa lysates A549 cells (IF)
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 29 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionInterconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 126.96.36.199 (synthase) and EC 188.8.131.52 (phosphatase), but with a reduced activity.
Tissue specificityIn mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues.
Involvement in diseaseDefects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10) [MIM:261670]. A metabolic disorder characterized by myoglobinuria, increased serum creatine kinase levels, decreased phosphoglycerate mutase activity, myalgia, muscle pain, muscle cramps and excercise intolerance.
Sequence similaritiesBelongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
- Information by UniProt
- BPG dependent PGAM 2 antibody
- BPG-dependent PGAM 2 antibody
- GSD10 antibody
All lanes : Anti-PGAM2 antibody (ab97800) at 1/10000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab97800 at 1/200 dilution. Lower image was also merged with DNA probe.
ab97800 has been referenced in 1 publication.
- Xu Y et al. Oxidative stress activates SIRT2 to deacetylate and stimulate phosphoglycerate mutase. Cancer Res 74:3630-42 (2014). PubMed: 24786789