Recombinant
RabMAb

Anti-PGAM2 antibody [EPR15193(B)] (ab183027)

Overview

  • Product name
    Anti-PGAM2 antibody [EPR15193(B)]
    See all PGAM2 primary antibodies
  • Description
    Rabbit monoclonal [EPR15193(B)] to PGAM2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PGAM2 aa 1-100. The exact sequence is proprietary.
    Database link: P15259

  • Positive control
    • Human skeletal muscle lysate; Human fetal heart lysate; Human skeletal muscle tissue; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR15193(B)
  • Isotype
    IgG

Associated products

Applications

Our Abpromise guarantee covers the use of ab183027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30 - 1/50.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/10000 - 1/50000. Predicted molecular weight: 29 kDa.

Target

  • Function
    Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.
  • Tissue specificity
    In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues.
  • Involvement in disease
    Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10) [MIM:261670]. A metabolic disorder characterized by myoglobinuria, increased serum creatine kinase levels, decreased phosphoglycerate mutase activity, myalgia, muscle pain, muscle cramps and excercise intolerance.
  • Sequence similarities
    Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • BPG dependent PGAM 2 antibody
    • BPG-dependent PGAM 2 antibody
    • GSD10 antibody
    • MGC88743 antibody
    • Muscle specific phosphoglycerate mutase antibody
    • Muscle-specific phosphoglycerate mutase antibody
    • OTTHUMP00000207787 antibody
    • PGAM 2 antibody
    • PGAM M antibody
    • PGAM-M antibody
    • Pgam2 antibody
    • PGAM2_HUMAN antibody
    • PGAMM antibody
    • phosphoglycerate mutase 2 (muscle) antibody
    • Phosphoglycerate mutase 2 antibody
    • Phosphoglycerate mutase isozyme M antibody
    see all

Images

  • All lanes : Anti-PGAM2 antibody [EPR15193(B)] (ab183027) at 1/20000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 29 kDa

  • Immunohistochemistry analysis of paraffin embedded Human skeletal muscle tissue, labeling PGAM2 with ab183027 at 1/500 dilution. Counterstained with Hematoxylin.

  • Immunopreciptation analysis of Human skletal muscle lysate, labeling PGAM2 with ab183027 at 1/50 dilution (lane 1) and negative control (lane 2)  Anti-Rabbit IgG (HRP) secondary antibody used at 1/1500 dilution (lane 1) and  blocking buffer was 5% NFDM/TBST.

  • Flow cytometry analysis of Jurkat cells fixed with 2% paraformaldehyde, labeling PGAM2 with ab183027 at 1/20 dilution.  Goat
    anti Rabbit IgG secondary antibody used at 1/150 dilution.

References

ab183027 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183027.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up