Overview

  • Product name
    Anti-PGAM5 antibody
    See all PGAM5 primary antibodies
  • Description
    Rabbit polyclonal to PGAM5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
  • Immunogen

    antigen sequence: NWDRREPLSL INVRKRNVES GEEELASKLD HYKAKATRHI FLIRHSQYHV DGSLEKDRTL TPLGREQAEL TGLRLASLGL KFNKIV, corresponding to amino acids 61-146 of Human PGAM5.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human uterus tissue; RT-4 and U-251 MG cell lysates; Human liver and tonsil lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
WB 1/500 - 1/1000. Predicted molecular weight: 32 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression.
  • Sequence similarities
    Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
  • Domain
    According to PubMed:18387606, may contain a non-cleaved N-terminal mitochondrial targeting sequence that targets PGAM5 to the cytosolic side of the outer mitochondrial membrane, instead of a N-terminal transmembrane.
  • Cellular localization
    Mitochondrion. Mitochondrion outer membrane. Membrane. Isoform 2 overexpression results in the formation of disconnected punctuate mitochondria distributed throughout the cytoplasm. Isoform 1 overexpression results in the clustering of mitochondria around the nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • Bcl-XL-binding protein v68 antibody
      • BXLBv68 antibody
      • MGC5352 antibody
      • mitochondrial antibody
      • PGAM5 antibody
      • PGAM5_HUMAN antibody
      • Phosphoglycerate mutase family member 5 antibody
      • Serine/threonine protein phosphatase PGAM5 mitochondrial antibody
      • Serine/threonine-protein phosphatase PGAM5 antibody
      see all

    Images

    • Western blot - Anti-PGAM5 antibody (ab126534)
      Western blot - Anti-PGAM5 antibody (ab126534)
      All lanes : Anti-PGAM5 antibody (ab126534) at 1/250 dilution

      Lane 1 : RT-4 cell lysate
      Lane 2 : U-251 MG cell lysate
      Lane 3 : Human plasma lysate
      Lane 4 : Human liver lysate
      Lane 5 : Human tonsil lysate

      Predicted band size: 32 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PGAM5 antibody (ab126534)
      Immunocytochemistry/ Immunofluorescence - Anti-PGAM5 antibody (ab126534)
      Immunofluorescent staining of Human cell line MCF-7 shows positivity in mitochondria. Recommended concentration of ab126534 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody (ab126534)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody (ab126534)
      ab126534, at 1/200 dilution, staining PGAM5 in paraffin-embedded Human uterus tissue by Immunohistochemistry.

    References

    This product has been referenced in:
    • Zhu P  et al. Ripk3 promotes ER stress-induced necroptosis in cardiac IR injury: A mechanism involving calcium overload/XO/ROS/mPTP pathway. Redox Biol 16:157-168 (2018). WB . Read more (PubMed: 29502045) »
    • Biswal S  et al. Salidroside mediated stabilization of Bcl -xL prevents mitophagy in CA3 hippocampal neurons during hypoxia. Neurobiol Dis 116:39-52 (2018). WB, IHC-Fr ; Rat . Read more (PubMed: 29723606) »

    See all 11 Publications for this product

    Publishing research using ab126534? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    5 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-PGAM5 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (thymus and spleen)
    Loading amount
    30 µg
    Specification
    thymus and spleen
    Gel Running Conditions
    Reduced Denaturing (10 Bis Tris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Read More
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    Abcam user community

    Verified customer

    Submitted Apr 18 2013

    Western blot abreview for Anti-PGAM5 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Xenopus laevis Tissue lysate - whole (whole embryo)
    Loading amount
    15 µg
    Specification
    whole embryo
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Nov 27 2012

    Xenopus: ab67992 und ab126534

    ab67992 : DISCOUNT CODE: ABTRIAL-000

    ab126534 : DISCOUNT CODE: ABTRIAL-000

    Ablaufdatum: 05. März 2013

    Es freut mich zu hören, dass Sie unser Angebot unsere Antikörper ab67992 und ab126534 in Xenopus zu teste...

    Read More


    Hola, Estoy interesado en usar el anticuerpo ab126534 con células procedentes de ratón. Sin embargo en vuestra datasheet solo figura Humano como especie testada. Como en la imagen del blot aparecen varias bandas inespecíficas, quisiera saber si po...

    Read More

    Gracias por contactarnos.



    El anticuerpo ab126534 solo se ha testado en nuestro laboratorio en muestras humanas, lo cual no quiere decir que no vaya a reconocer la proteína en otras especies, pero al no tener datos para probar...

    Read More

    Does this antibody recognize both isoforms of PGAM5 oris one of the bands around 32kDa non-specific?

    Thank you for contacting Abcam regarding ab126534.


    The Anti-PGAM5 antibody can recognize both the PGAM5-001 (ENSP00000321503) and PGAM5-005 (ENSP00000438465) isoforms, since the antigen sequence has 100% sequence identity to these two tra...

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