Anti-PGAM5 antibody (ab126534)

Reviews (2)Q&A (3)References (15)

Overview

  • Product name
    Anti-PGAM5 antibody
    See all PGAM5 primary antibodies
  • Description
    Rabbit polyclonal to PGAM5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
  • Immunogen

    Recombinant fragment corresponding to Human PGAM5 aa 61-146.
    Sequence:

    NWDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQYHV DGSLEKDRTLTPLGREQAELTGLRLASLGLKFNKIV


    Database link: Q96HS1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • ICC/IF: MCF-7 cells WB: RT-4 cell transfected with siRNA IHC-P: Human liver, testis, tonsil and colon tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
WB 1/500 - 1/1000. Predicted molecular weight: 32 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression.
  • Sequence similarities
    Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
  • Domain
    According to PubMed:18387606, may contain a non-cleaved N-terminal mitochondrial targeting sequence that targets PGAM5 to the cytosolic side of the outer mitochondrial membrane, instead of a N-terminal transmembrane.
  • Cellular localization
    Mitochondrion. Mitochondrion outer membrane. Membrane. Isoform 2 overexpression results in the formation of disconnected punctuate mitochondria distributed throughout the cytoplasm. Isoform 1 overexpression results in the clustering of mitochondria around the nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • Bcl-XL-binding protein v68 antibody
      • BXLBv68 antibody
      • MGC5352 antibody
      • mitochondrial antibody
      • PGAM5 antibody
      • PGAM5_HUMAN antibody
      • Phosphoglycerate mutase family member 5 antibody
      • Serine/threonine protein phosphatase PGAM5 mitochondrial antibody
      • Serine/threonine-protein phosphatase PGAM5 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody (ab126534)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody (ab126534)
      Immunohistochemical analysis of human colon tissue labeling PGAM5 with ab126534 at 1/200 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody (ab126534)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody (ab126534)
      Immunohistochemical analysis of human tonsil tissue labeling PGAM5 with ab126534 at 1/200 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody (ab126534)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody (ab126534)
      Immunohistochemical analysis of human testis tissue labeling PGAM5 with ab126534 at 1/200 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody (ab126534)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody (ab126534)
      Immunohistochemical analysis of human liver tissue labeling PGAM5 with ab126534 at 1/200 dilution.
    • Western blot - Anti-PGAM5 antibody (ab126534)
      Western blot - Anti-PGAM5 antibody (ab126534)
      All lanes : Anti-PGAM5 antibody (ab126534) at 0.4 µg/ml

      Lane 1 : RT-4 cell transfected with control siRNA, 100%
      Lane 2 : RT-4 cell transfected with siRNA Probe 1, 50%
      Lane 3 : RT-4 cell transfected with siRNA probe 2, 45%

      Predicted band size: 32 kDa



      Loading control: Anti-PPIB

      Genetic validation in WB by siRNA knockdown.

    • Immunocytochemistry/ Immunofluorescence - Anti-PGAM5 antibody (ab126534)
      Immunocytochemistry/ Immunofluorescence - Anti-PGAM5 antibody (ab126534)
      Immunofluorescent staining of Human cell line MCF-7 shows positivity in mitochondria. Recommended concentration of ab126534 1-4 µg/ml. Cells treated with PFA/Triton X-100.

    References

    This product has been referenced in:
    • Zhu P  et al. Ripk3 promotes ER stress-induced necroptosis in cardiac IR injury: A mechanism involving calcium overload/XO/ROS/mPTP pathway. Redox Biol 16:157-168 (2018). WB . Read more (PubMed: 29502045) »
    • Biswal S  et al. Salidroside mediated stabilization of Bcl -xL prevents mitophagy in CA3 hippocampal neurons during hypoxia. Neurobiol Dis 116:39-52 (2018). WB, IHC-Fr ; Rat . Read more (PubMed: 29723606) »
    See all 15 Publications for this product

    Publishing research using ab126534? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-PGAM5 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (thymus and spleen)
    Loading amount
    30 µg
    Specification
    thymus and spleen
    Gel Running Conditions
    Reduced Denaturing (10 Bis Tris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 18 2013

    Western blot abreview for Anti-PGAM5 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Xenopus laevis Tissue lysate - whole (whole embryo)
    Loading amount
    15 µg
    Specification
    whole embryo
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 27 2012

    Question

    Xenopus: ab67992 und ab126534

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    Answer

    ab67992 : DISCOUNT CODE: ABTRIAL-000

    ab126534 : DISCOUNT CODE: ABTRIAL-000

    Ablaufdatum: 05. März 2013

    Es freut mich zu hören, dass Sie unser Angebot unsere Antikörper ab67992 und ab126534 in Xenopus zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincodes zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Die Discountcodes decken je den Wert von ab67992 oder ab126534 ab und können zum Kauf weiterer Produkte eingesetzt werden.

    Ein Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in Xenopus eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code.

    Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

    Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

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    Question


    Hola, Estoy interesado en usar el anticuerpo ab126534 con células procedentes de ratón. Sin embargo en vuestra datasheet solo figura Humano como especie testada. Como en la imagen del blot aparecen varias bandas inespecíficas, quisiera saber si podéis enviarme una muestra de prueba para testar el anticuerpo con nuestras muestras.
    Muchas gracias.

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    Answer

    Gracias por contactarnos.



    El anticuerpo ab126534 solo se ha testado en nuestro laboratorio en muestras humanas, lo cual no quiere decir que no vaya a reconocer la proteína en otras especies, pero al no tener datos para probarlo, no podemos garantizar su uso. Sin embargo, he llevado a cabo una alineación de secuencias entre el inmunógeno del anticuerpo y la proteína de ratón, y el porcentaje es muy elevado (94%):




    Sequence type explicitly set to Protein

    Sequence format is Pearson

    Sequence 1: IMMUNOGEN 86 aa

    Sequence 2: MOUSE 288 aa

    Start of Pairwise alignments

    Aligning...







    Sequences (1:2) Aligned. Score: 94.186

    Guide tree file created: http://www.genome.jp/tools-bin/pushfile?121019171842ErF4C+clustalw.dnd



    There are 1 groups

    Start of Multiple Alignment



    Aligning...

    Group 1: Sequences: 2 Score:1371

    Alignment Score 485



    CLUSTAL-Alignment file created http://www.genome.jp/tools-bin/pushfile?121019171842ErF4C+clustalw.aln






    http://www.genome.jp/tools-bin/pushfile?121019171842ErF4C+clustalw.aln

    CLUSTAL 2.1 multiple sequence alignment





    IMMUNOGEN -----------------------------------------------------------N

    MOUSE MAFRQALQLAACGLAGGSAAVLFSAVAVGKPRGGGDADTRATEPPAWTGARAGRGVWDTN
    *



    IMMUNOGEN WDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQYHVDGSLEKDRTLT

    MOUSE WDRREPLSLINLKKRNVESGEDELTSRLDHYKAKATRHIFLIRHSQYHVDGSLEKDRTLT
    ***********::********:**:*:*********************************



    IMMUNOGEN PLGREQAELTGLRLASLGLKFNKIV-----------------------------------

    MOUSE PLGREQAELTGLRLASLGLKFNKIVHSSMTRAVETTDIISKHLPGVSRVSTDLLREGAPI
    *************************



    IMMUNOGEN ------------------------------------------------------------

    MOUSE EPDPPVSHWKPEAVQYYEDGARIEAAFRNYIHRADARQEEDSYEIFICHANVIRYIVCRA




    IMMUNOGEN ------------------------------------------------

    MOUSE LQFPPEGWLRLSLNNGSITHLVIRPNGRVALRTLGDTGFMPPDKITRS






    En Abcam ofrecemos un servicio de evaluación de anticuerpos, llamado Abreview, por el cual si compráis un anticuerpo y lo testáis en una nueva especie o aplicación y nos enviáis los resultados a la web (en forma de Abreview) os ofrecemos un descuento para la futura compra por el valor del anticuerpo testado (€399.00 en este caso).



    Si te interesa esta oferta, sigue los siguientes pasos:

    1. Contesta a este mail indicando que le gustaría probar en muestras de ratón. En ese momento se te enviará un código de descuento. Dicho código debe emitirse antes de la compra del producto en cuestión, así que te ruego que esperes a recibir una respuesta antes de hacer el pedido.

    2. Realiza la compra del anticuerpo bien sea por teléfono, fax, o a través de nuestra web (www.abcam.com)

    3. Pruébalo en la nueva especie a testar.

    4. A través del sistema Abreview, comparte tus resultados con nosotros, tanto si son positivos, como negativos (este proceso puede durar unos 10 minutos. Valoramos enormemente si pudieras adjuntar imágenes al Abreview!).

    5. Una vez hayamos publicado tu reseña, el código de descuento se activa. Simplemente realiza el nuevo pedido por teléfono, fax, o a través de la web, y no olvides mencionar el código de descuento. El descuento es válido durante 4 meses una vez emitido.

    Estamos muy agradecidos de recibir feedback de nuestros productos y cualquier información que puedas facilitarnos es bienvenida. Incluso si el producto resulta inadecuado para la especie testada, recibirás igualmente el descuento en tu próxima compra una vez que el Abreview haya sido publicado.

    Para conocer con más detalle nuestro sistema de Abreview, visita la página: https://www.abcam.com/abreview.

    No dudes en ponerte en contacto con nosotros si tienes cualquier otra consulta sobre esta oferta y estaré encantada de poder ayudarte.

    Las condiciones de uso de esta oferta puede usted consultarlas en https://www.abcam.com/collaborationdiscount.

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    Question

    Does this antibody recognize both isoforms of PGAM5 oris one of the bands around 32kDa non-specific?

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    Answer

    Thank you for contacting Abcam regarding ab126534.


    The Anti-PGAM5 antibody can recognize both the PGAM5-001 (ENSP00000321503) and PGAM5-005 (ENSP00000438465) isoforms, since the antigen sequence has 100% sequence identity to these two transcripts.

    The molecular weight of the two transcripts, 28 and 32 kDa, matches the two bands on the blot between the 28 and 36 kDa markers according to our QC criteria, so I have no reason to believe that either of the two bands should be unspecific.

    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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