Recombinant Anti-PGBD1 antibody [EPR13883] - BSA and Azide free (ab250242)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13883] to PGBD1 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PGBD1 antibody [EPR13883] - BSA and Azide free
See all PGBD1 primary antibodies -
Description
Rabbit monoclonal [EPR13883] to PGBD1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250242 is the carrier-free version of ab180598.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13883 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa). |
Target
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Sequence similarities
Contains 1 SCAN box domain. - Information by UniProt
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Database links
- Clone ID: 103694097 Rat
- Entrez Gene: 84547 Human
- Entrez Gene: 319207 Mouse
- SwissProt: Q96JS3 Human
- Unigene: 144527 Human
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Alternative names
- Cerebral protein 4 antibody
- dJ874C20.4 antibody
- HUCEP 4 antibody
see all
Images
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This data was developed using ab180598, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling PGBD1 with ab180598 at a 1/500 dilution (left image), and counterstained with Dapi (right image). Goat anti rabbit IgG (Dylight 488) secondary used at a 1/200 diution
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This data was developed using ab180598, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of HeLa cells using ab180598 at a 1/20 dilution (red) or a rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
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All lanes : Anti-PGBD1 antibody [EPR13883] (ab180598) at 1/10000 dilution
Lane 1 : HeLa lysate
Lane 2 : Jurkat lysate
Lane 3 : 293 lysate
Lane 4 : K562 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated at 1/1000 dilution
Predicted band size: 93 kDaThis data was developed using ab180598, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250242 has not yet been referenced specifically in any publications.