Recombinant
RabMAb

Recombinant Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)

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Overview

  • Product name

    Anti-PGC1 alpha + beta antibody [EPR18289]
    See all PGC1 alpha + beta primary antibodies

  • Description

    Rabbit monoclonal [EPR18289] to PGC1 alpha + beta

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PGC1 alpha + beta aa 750 to the C-terminus. The exact sequence is proprietary. SwissProt of PGC1 beta is Q86YN6.
    Database link: Q9UBK2

  • Positive control

    • WB: Recombinant fragment of Human PGC1 alpha protein; Recombinant fragment of Human PGC1 beta protein; Human skeletal muscle lysate, HeLa cell lysate and mouse colon lysate; Human fetal heart lysate, Human fetal kidney lysate, Mouse thymus lysate and Rat thymus lysate; Mouse brain and heart lysates; Rat brain and heart lysates; C6 and NIH/3T3 cell lysates; Mouse kidney lysate and rat kidney lysate; Human skeletal muscle lysate.

  • General notes

    This product may be covered by one or more of the following U.S. Patents.

    6,166,192
    6,426,411
    6,525,178
    6,872,391
    6,908,987
    7,250,283
    7,091,006
    7,354,738
    8,105,774
    8,445,233

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab188102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 91, 113 kDa).

Target

  • Relevance

    PGC1 alpha - The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene). PGC1 beta - Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure.

  • Cellular localization

    Nucleus

  • Database links

    see all

  • Alternative names

    • LEM6 antibody
    • Ligand effect modulator 6 antibody
    • PERC antibody
    • Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha antibody
    • Peroxisome proliferative activated receptor, gamma, coactivator 1 antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha antibody
    • peroxisome proliferator-activated receptor gamma coactivator 1 beta antibody
    • Peroxisome proliferator-activated receptor gamma coactivator 1-alpha antibody
    • Peroxisome proliferator-activated receptor gamma coactivator 1-beta antibody
    • peroxisome proliferator-activated receptor gamma, coactivator 1 beta antibody
    • PGC 1 (alpha) antibody
    • PGC 1 alpha antibody
    • PGC 1v antibody
    • PGC-1(beta) antibody
    • PGC-1-alpha antibody
    • PGC-1-beta antibody
    • PGC-1-related estrogen receptor alpha coactivator antibody
    • PGC1 antibody
    • PGC1(alpha) antibody
    • PGC1A antibody
    • PGC1v antibody
    • PPAR gamma coactivator 1 alpha antibody
    • PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6 antibody
    • PPAR gamma coactivator 1 antibody
    • PPAR gamma coactivator variant form antibody
    • PPAR gamma coactivator-1beta antibody
    • PPAR-gamma coactivator 1-alpha antibody
    • PPAR-gamma coactivator 1-beta antibody
    • PPARGC 1 alpha antibody
    • PPARGC-1-alpha antibody
    • PPARGC-1-beta antibody
    • PPARGC1 antibody
    • PPARGC1A antibody
    • Ppargc1b antibody
    • PRGC1_HUMAN antibody
    • PRGC2_HUMAN antibody
    see all

Images

  • Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
    Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
    Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/100000 dilution + Recombinant fragment of Human PGC1 alpha protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 91, 113 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    5% NFDM/TBST: Blocking and diluting buffer.

    Recombinant fragment of human PGC1 alpha protein contains aa459-798 with His-Tag®.

  • Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
    Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
    Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/100000 dilution + Recombinant fragment of Human PGC1 beta protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 91, 113 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

    Recombinant fragment of human PGC1 beta protein contains aa751-1023 with His-Tag®.

  • Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
    Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
    All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Mouse colon lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 91, 113 kDa
    Observed band size: 90, 113 kDa why is the actual band size different from the predicted?



    5% NFDM/TBST: Blocking and diluting buffer.

    The composite image shows duplicate western blots with different exposure times. A 90 KDa band (arrow), likely to be PGC1 alpha, is detected at a longer exposure time (1 min). It has been reported that the skeletal muscle, heart and kidney have a higher expression level of PGC1 alpha than other tissues (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925).

  • Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
    Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
    All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/10000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Mouse thymus lysate
    Lane 4 : Rat thymus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 91, 113 kDa
    Observed band size: 113 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
    Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
    All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/10000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat heart lysate
    Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 6 : NIH 3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 91, 113 kDa
    Observed band size: 113 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
    Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
    All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 91, 113 kDa
    Observed band size: 113 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

    A high expression level of PGC1 alpha in the kidney has been reported (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925). The lower band in lane 2 is likely to be the alpha form.

  • Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
    Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
    All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1000 µg

    Predicted band size: 91, 113 kDa
    Observed band size: 113 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    5% NFDM/TBST: Blocking and diluting buffer.

    A high expression level of PGC1 alpha in the kidney has been reported (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925). The lower band in lane 2 is likely to be the alpha form.

References

ab188102 has not yet been referenced specifically in any publications.

Publishing research using ab188102? Please let us know so that we can cite the reference in this datasheet.

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