Recombinant Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
![Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)](https://www.abcam.com/ps/products/188/ab188102/Images/ab188102-248828-anti-pgc1-alpha-beta-antibody-epr18289-western-blot.jpg "Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18289] to PGC1 alpha + beta
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-PGC1 alpha + beta antibody [EPR18289]
See all PGC1 alpha + beta primary antibodies -
Description
Rabbit monoclonal [EPR18289] to PGC1 alpha + beta -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PGC1 alpha + beta aa 750 to the C-terminus. The exact sequence is proprietary. SwissProt of PGC1 beta is Q86YN6.
Database link: Q9UBK2 -
Positive control
- WB: Recombinant fragment of Human PGC1 alpha protein; Recombinant fragment of Human PGC1 beta protein; Human skeletal muscle lysate, HeLa cell lysate and mouse colon lysate; Human fetal heart lysate, Human fetal kidney lysate, Mouse thymus lysate and Rat thymus lysate; Mouse brain and heart lysates; Rat brain and heart lysates; C6 and NIH/3T3 cell lysates; Mouse kidney lysate and rat kidney lysate; Human skeletal muscle lysate.
-
General notes
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18289 -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Associated products
-
Alternative Versions
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab188102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 91, 113 kDa). |
Target
-
Relevance
PGC1 alpha - The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene). PGC1 beta - Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. -
Cellular localization
Nucleus -
Database links
- Entrez Gene: 10891 Human
- Entrez Gene: 133522 Human
- Entrez Gene: 170826 Mouse
- Entrez Gene: 19017 Mouse
- Entrez Gene: 291567 Rat
- Entrez Gene: 83516 Rat
- Omim: 604517 Human
- Omim: 608886 Human
see all -
Alternative names
- LEM6 antibody
- Ligand effect modulator 6 antibody
- PERC antibody
see all
Images
-
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/100000 dilution + Recombinant fragment of Human PGC1 alpha protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 91, 113 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute5% NFDM/TBST: Blocking and diluting buffer.
Recombinant fragment of human PGC1 alpha protein contains aa459-798 with His-Tag®.
-
![Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)](https://www.abcam.com/ps/products/188/ab188102/Images/ab188102-248827-anti-pgc1-alpha-beta-antibody-epr18289-western-blot.jpg)
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/100000 dilution + Recombinant fragment of Human PGC1 beta protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 91, 113 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds5% NFDM/TBST: Blocking and diluting buffer.
Recombinant fragment of human PGC1 beta protein contains aa751-1023 with His-Tag®.
-
![Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)](https://www.abcam.com/ps/products/188/ab188102/Images/ab188102-248826-anti-pgc1-alpha-beta-antibody-epr18289-western-blot.jpg)
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 3 : Mouse colon lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 91, 113 kDa
Observed band size: 90, 113 kDa why is the actual band size different from the predicted?5% NFDM/TBST: Blocking and diluting buffer.
The composite image shows duplicate western blots with different exposure times. A 90 KDa band (arrow), likely to be PGC1 alpha, is detected at a longer exposure time (1 min). It has been reported that the skeletal muscle, heart and kidney have a higher expression level of PGC1 alpha than other tissues (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925).
-
![Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)](https://www.abcam.com/ps/products/188/ab188102/Images/ab188102-248825-anti-pgc1-alpha-beta-antibody-epr18289-western-blot.jpg)
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/10000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Mouse thymus lysate
Lane 4 : Rat thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes5% NFDM/TBST: Blocking and diluting buffer.
-
![Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)](https://www.abcam.com/ps/products/188/ab188102/Images/ab188102-248824-anti-pgc1-alpha-beta-antibody-epr18289-western-blot.jpg)
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/10000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
Lane 6 : NIH 3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute5% NFDM/TBST: Blocking and diluting buffer.
-
![Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)](https://www.abcam.com/ps/products/188/ab188102/Images/ab188102-248822-anti-pgc1-alpha-beta-antibody-epr18289-western-blot.jpg)
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds5% NFDM/TBST: Blocking and diluting buffer.
A high expression level of PGC1 alpha in the kidney has been reported (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925). The lower band in lane 2 is likely to be the alpha form.
-
![Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)](https://www.abcam.com/ps/products/188/ab188102/Images/ab188102-248821-anti-pgc1-alpha-beta-antibody-epr18289-western-blot.jpg)
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1000 µg
Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute5% NFDM/TBST: Blocking and diluting buffer.
A high expression level of PGC1 alpha in the kidney has been reported (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925). The lower band in lane 2 is likely to be the alpha form.
-
![Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)](https://www.abcam.com/ps/products/188/ab188102/Images/ab188102-8-benefits-of-recombinant-antibodies.png)
Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
Datasheets and documents
Certificate of Compliance
References (0)
ab188102 has not yet been referenced specifically in any publications.
