This product may be covered by one or more of the following U.S. Patents.
Our Abpromise guarantee covers the use of ab54481 in the following tested applications.
|WB||1/1000. Predicted molecular weight: 92 kDa.
Use of nuclear extracts for WB is recommended since protein is nuclear and expression within whole cell lysate can be low.
|ICC/IF||1/300. PubMed: 22545021|
|IHC-P||Use at an assay dependent concentration.|
Immunofluorescence analysis of Human white adipose tissue progenitor cells, staining PGC1 alpha (green) with ab54481.
Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 for 5 min. Cells were incubated with primary antibody (1/300) and staining was detected with Alexa Fluor® 546-conjugated anti-rabbit secondary antibody (1/1000).
Western blot using ab54481 at 1:2000 to detect PGC1 alpha in Mouse brown adipose tissue whole cell extract (both lanes).