Western blot abreview for Anti-PGC1 alpha antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (human fibroblast)
Loading amount
50 µg
Specification
human fibroblast
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST

Secondary antibody

Name
Non-Abcam antibody was used: HRP anti-mouse
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 100 kDa Non-specific: 50, 40 kDa

Additional data

Abcam response
Protcol optimization might be necessory for this anibody.

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Submitted Nov 01 2012

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