Immunocytochemistry/ Immunofluorescence abreview for Anti-PGC1 alpha antibody

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Cardiomyocytes)
Permeabilization
Yes - Triton X-100
Specification
Cardiomyocytes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Serum in PBS
Dilution
1/50

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Donkey anti-Mouse 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
The staining pattern is incorrect. PGC1A is located in the nucleus. (This is well known and is clearly shown in reviews of your other PGC1A antibodies). The staining pattern I detected is not.
Abcam response
We would like to thank the reviewer for submitting an Abreview for this product. As the product had not yet been tested in ICC/IF applications with this sample type, we were unsure what the result might be; we appreciate the time and effort that the reviewer has put in to testing this product in this application. Further optimization may be necessary for use of this product in ICC/IF with this sample type, and we are happy to help troubleshoot any products or applications which may not be performing. We do encourage all of our customers to contact us should they wish to test any of our products in untested species or applications, as those products may qualify for our AbTrial Testing Program.

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Submitted Sep 28 2016

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