Anti-PGC1 alpha antibody - N-terminal (ab191838)
Key features and details
- Rabbit polyclonal to PGC1 alpha - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PGC1 alpha antibody - N-terminal
See all PGC1 alpha primary antibodies -
Description
Rabbit polyclonal to PGC1 alpha - N-terminal -
Host species
Rabbit -
Specificity
At least three isoforms of PGC1 alpha are known to exist; this antibody will detect all three isoforms. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
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Immunogen
Synthetic peptide within Human PGC1 alpha (N terminal). The exact sequence is proprietary. The immunogen is located within amino acids 200 - 250 of PGC1 alpha and consists of a 17 amino acid peptide.
Database link: Q9UBK2 -
Positive control
- WB: Human heart lysate. IHC-P: Rat heart tissue.
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General notes
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab191838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 91 kDa. Blocking in 5% non-fat milk in TBS at 4 overnight. ab191838 incubation at room temperature for 1 hour. |
|
| IHC-P | Use a concentration of 5 - 20 µg/ml. | |
| ICC/IF | Use a concentration of 20 µg/ml. |
Target
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Function
Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK. -
Tissue specificity
Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.
Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.
Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus; Nucleus and Nucleus. Nucleus, PML body. - Information by UniProt
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Database links
- Entrez Gene: 338446 Cow
- Entrez Gene: 10891 Human
- Entrez Gene: 19017 Mouse
- Entrez Gene: 497232 Pig
- Entrez Gene: 83516 Rat
- Omim: 604517 Human
- SwissProt: Q865B7 Cow
- SwissProt: Q9UBK2 Human
see all -
Alternative names
- L PGC 1alpha antibody
- LEM6 antibody
- Ligand effect modulator 6 antibody
see all
Images
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Western blot - Anti-PGC1 alpha antibody - N-terminal (ab191838)All lanes : Anti-PGC1 alpha antibody - N-terminal (ab191838) at 1 µg/ml
Lane 1 : Human heart tissue lysate
Lane 2 : Human heart tissue lysate with Blocking peptide
Predicted band size: 91 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGC1 alpha antibody - N-terminal (ab191838)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of rat heart tissue labeling PGC1 alpha with Anti-PGC1 alpha antibody - N-terminal (ab191838) at 5μg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha antibody - N-terminal (ab191838)Immunofluorescent analysis of rat heart tissue labeling PGC1 alpha with ab191838 at 20 µg/ml
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGC1 alpha antibody - N-terminal (ab191838)Immunohistochemical analysis of rat heart tissue labeling PGC1 alpha with ab191838 at 5 µg/ml.
Protocols
References (17)
ab191838 has been referenced in 17 publications.
- Varghese MV et al. Antioxidant-Conjugated Peptide Attenuated Metabolic Reprogramming in Pulmonary Hypertension. Antioxidants (Basel) 9:N/A (2020). PubMed: 31991719
- Gao Y et al. Role of Parkin-mediated mitophagy in the protective effect of polydatin in sepsis-induced acute kidney injury. J Transl Med 18:114 (2020). PubMed: 32131850
- Mishra JS et al. Testosterone Decreases Placental Mitochondrial Content and Cellular Bioenergetics. Biology (Basel) 9:N/A (2020). PubMed: 32698476
- Wen J et al. Cardioprotective effects of Aconiti Lateralis Radix Praeparata combined with Zingiberis Rhizoma on doxorubicin-induced chronic heart failure in rats and potential mechanisms. J Ethnopharmacol 238:111880 (2019). PubMed: 31004728
- Brown LA et al. Late life maintenance and enhancement of functional exercise capacity in low and high responding rats after low intensity treadmill training. Exp Gerontol 125:110657 (2019). PubMed: 31306740
