Recombinant Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18289] to PGC1 alpha + beta
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-PGC1 alpha + beta antibody [EPR18289]
See all PGC1 alpha + beta primary antibodies -
Description
Rabbit monoclonal [EPR18289] to PGC1 alpha + beta -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Recombinant fragment of Human PGC1 alpha protein; Recombinant fragment of Human PGC1 beta protein; Human skeletal muscle lysate, HeLa cell lysate and mouse colon lysate; Human fetal heart lysate, Human fetal kidney lysate, Mouse thymus lysate and Rat thymus lysate; Mouse brain and heart lysates; Rat brain and heart lysates; C6 and NIH/3T3 cell lysates; Mouse kidney lysate and rat kidney lysate; Human skeletal muscle lysate.
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General notes
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18289 -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 91, 113 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 91, 113 kDa). |
Target
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Relevance
PGC1 alpha - The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene). PGC1 beta - Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. -
Cellular localization
Nucleus -
Database links
- Entrez Gene: 10891 Human
- Entrez Gene: 133522 Human
- Entrez Gene: 170826 Mouse
- Entrez Gene: 19017 Mouse
- Entrez Gene: 291567 Rat
- Entrez Gene: 83516 Rat
- Omim: 604517 Human
- Omim: 608886 Human
see all -
Alternative names
- LEM6 antibody
- Ligand effect modulator 6 antibody
- PERC antibody
see all
Images
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All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/10000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute5% NFDM/TBST: Blocking and diluting buffer.
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Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/100000 dilution + Recombinant fragment of Human PGC1 alpha protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 91, 113 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute5% NFDM/TBST: Blocking and diluting buffer.
Recombinant fragment of human PGC1 alpha protein contains aa459-798 with His-Tag®.
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Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/100000 dilution + Recombinant fragment of Human PGC1 beta protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 91, 113 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds5% NFDM/TBST: Blocking and diluting buffer.
Recombinant fragment of human PGC1 beta protein contains aa751-1023 with His-Tag®.
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All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution
Lane 1 : Human skeletal muscle tissue lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 3 : Mouse colon tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 91, 113 kDa
Observed band size: 90, 113 kDa why is the actual band size different from the predicted?5% NFDM/TBST: Blocking and diluting buffer.
The composite image shows duplicate western blots with different exposure times. A 90 KDa band (arrow), likely to be PGC1 alpha, is detected at a longer exposure time (1 min). It has been reported that the skeletal muscle, heart and kidney have a higher expression level of PGC1 alpha than other tissues (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925).
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All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/10000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : Mouse thymus tissue lysate
Lane 4 : Rat thymus tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes5% NFDM/TBST: Blocking and diluting buffer.
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All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds5% NFDM/TBST: Blocking and diluting buffer.
A high expression level of PGC1 alpha in the kidney has been reported (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925). The lower band in lane 2 is likely to be the alpha form.
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All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1000 µg
Predicted band size: 91, 113 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute5% NFDM/TBST: Blocking and diluting buffer.
A high expression level of PGC1 alpha in the kidney has been reported (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925). The lower band in lane 2 is likely to be the alpha form.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab188102 has been referenced in 5 publications.
- Li J et al. Dietary L-carnitine regulates liver lipid metabolism via simultaneously activating fatty acid β-oxidation and suppressing endoplasmic reticulum stress in large yellow croaker fed with high-fat diets. Br J Nutr N/A:1-34 (2022). PubMed: 35473947
- Xiao Z et al. MicroRNA-205-5p plays a suppressive role in the high-fat diet-induced atrial fibrosis through regulation of the EHMT2/IGFBP3 axis. Genes Nutr 17:11 (2022). PubMed: 35858845
- Zhang Q et al. Neuroprotective Effect of miR-483-5p Against Cardiac Arrest-Induced Mitochondrial Dysfunction Mediated Through the TNFSF8/AMPK/JNK Signaling Pathway. Cell Mol Neurobiol N/A:N/A (2022). PubMed: 36266523
- Kim HJ et al. Resistance exercise training-induced skeletal muscle strength provides protective effects on high-fat-diet-induced metabolic stress in mice. Lab Anim Res 38:36 (2022). PubMed: 36461131
- Zhou M et al. Acupoint catgut embedding improves senescence in a rat model of ageing by regulating mitophagy via the PINK1 pathway. J Cell Mol Med 25:3816-3828 (2021). PubMed: 33645011