Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PGC1 alpha + beta aa 750 to the C-terminus. The exact sequence is proprietary. SwissProt of PGC1 beta is Q86YN6. Database link: Q9UBK2
WB: Recombinant fragment of Human PGC1 alpha protein; Recombinant fragment of Human PGC1 beta protein; Human skeletal muscle lysate, HeLa cell lysate and mouse colon lysate; Human fetal heart lysate, Human fetal kidney lysate, Mouse thymus lysate and Rat thymus lysate; Mouse brain and heart lysates; Rat brain and heart lysates; C6 and NIH/3T3 cell lysates; Mouse kidney lysate and rat kidney lysate; Human skeletal muscle lysate.
This product may be covered by one or more of the following U.S. Patents.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 91, 113 kDa).
PGC1 alpha - The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene).
PGC1 beta - Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure.
The composite image shows duplicate western blots with different exposure times. A 90 KDa band (arrow), likely to be PGC1 alpha, is detected at a longer exposure time (1 min). It has been reported that the skeletal muscle, heart and kidney have a higher expression level of PGC1 alpha than other tissues (Kressler et al. 2002, J. Biol. Chem. 277, 13918–13925).
Western blot - Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102)
All lanes : Anti-PGC1 alpha + beta antibody [EPR18289] (ab188102) at 1/10000 dilution
Lane 1 : Human fetal heart lysate Lane 2 : Human fetal kidney lysate Lane 3 : Mouse thymus lysate Lane 4 : Rat thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution