Overview

  • Product name
    Anti-PGC1 beta antibody [EPR12370]
    See all PGC1 beta primary antibodies
  • Description
    Rabbit monoclonal [EPR12370] to PGC1 beta
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PGC1 beta aa 950 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q86YN6

  • Positive control
    • WB: HeLa, MCF-7 and SH-SY5Y cell lysates and human fetal brain, human fetal heart, rat spleen and mouse spleen tissue lysates. IP: HeLa whole cell lysate (ab150035).
  • General notes

    This product may be covered by one or more of the following U.S. Patents.

    6,166,192
    6,426,411
    6,525,178
    6,872,391
    6,908,987
    7,250,283
    7,091,006
    7,354,738
    8,105,774
    8,445,233 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab176328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 113 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner.
    • Tissue specificity
      Ubiquitous with higher expression in heart, brain and skeletal muscle.
    • Sequence similarities
      Contains 1 RRM (RNA recognition motif) domain.
    • Domain
      Contains 2 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which are usually required for the association with nuclear receptors.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • PERC antibody
      • peroxisome proliferative activated receptor, gamma, coactivator 1, antibody
      • peroxisome proliferator-activated receptor gamma coactivator 1 beta antibody
      • Peroxisome proliferator-activated receptor gamma coactivator 1-beta antibody
      • peroxisome proliferator-activated receptor gamma, coactivator 1 beta antibody
      • PGC-1(beta) antibody
      • PGC-1-beta antibody
      • PGC-1-related estrogen receptor alpha coactivator antibody
      • PGC1 antibody
      • PPAR gamma coactivator-1beta antibody
      • PPAR-gamma coactivator 1-beta antibody
      • PPARGC-1-beta antibody
      • PPARGC1 antibody
      • Ppargc1b antibody
      • PRGC2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/10000 dilution (purified)

      Lane 1 : HeLa whole cell lysate
      Lane 2 : MCF-7 whole cell lysate
      Lane 3 : SH-SY5Y whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 113 kDa
      Observed band size: 113 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.
    • All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/10000 dilution (purified)

      Lane 1 : Mouse spleen tissue lysate
      Lane 2 : Rat spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 113 kDa
      Observed band size: 113 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.
    • ab176328 (purified) at 1/20 immunoprecipitating PGC1 beta in HeLa whole cell lysate.

      Lane 1 (input): HeLa whole cell lysate (10µg)

      Lane 2 (+): ab176328 + HeLa whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab176328 in HeLa whole cell lysate.

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : Human fetal brain tissue lysate
      Lane 4 : Human fetal heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 113 kDa

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling PGC1 beta using unpurified ab176328 at a dilution of 1/10.

    References

    This product has been referenced in:
    • Mikó E  et al. Lithocholic acid, a bacterial metabolite reduces breast cancer cell proliferation and aggressiveness. Biochim Biophys Acta N/A:N/A (2018). Read more (PubMed: 29655782) »
    • Lu Y  et al. Mitophagy is required for brown adipose tissue mitochondrial homeostasis during cold challenge. Sci Rep 8:8251 (2018). Read more (PubMed: 29844467) »
    See all 9 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human hepatocytes, HepG2)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    human hepatocytes, HepG2
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Apr 24 2017

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Kidney)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    Kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Wesley Konsavage

    Verified customer

    Submitted Dec 09 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Permeabilization
    Yes - PBS 1X / 0.5% Triton X100
    Specification
    skin
    Blocking step
    PBS1X / 3% BSA / 10% NGS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Acetone

    Abcam user community

    Verified customer

    Submitted Aug 12 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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