Recombinant
RabMAb

Recombinant Anti-PGC1 beta antibody [EPR12370] - BSA and Azide free (ab240188)

Overview

  • Product name

    Anti-PGC1 beta antibody [EPR12370] - BSA and Azide free
    See all PGC1 beta primary antibodies
  • Description

    Rabbit monoclonal [EPR12370] to PGC1 beta - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PGC1 beta aa 950 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q86YN6

  • General notes

    ab240188 is the carrier-free version of ab176328 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240188 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product may be covered by one or more of the following U.S. Patents.

    6,166,192
    6,426,411
    6,525,178
    6,872,391
    6,908,987
    7,250,283
    7,091,006
    7,354,738
    8,105,774
    8,445,233

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab240188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 113 kDa.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner.
    • Tissue specificity

      Ubiquitous with higher expression in heart, brain and skeletal muscle.
    • Sequence similarities

      Contains 1 RRM (RNA recognition motif) domain.
    • Domain

      Contains 2 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which are usually required for the association with nuclear receptors.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • PERC antibody
      • peroxisome proliferative activated receptor, gamma, coactivator 1, antibody
      • peroxisome proliferator-activated receptor gamma coactivator 1 beta antibody
      • Peroxisome proliferator-activated receptor gamma coactivator 1-beta antibody
      • peroxisome proliferator-activated receptor gamma, coactivator 1 beta antibody
      • PGC-1(beta) antibody
      • PGC-1-beta antibody
      • PGC-1-related estrogen receptor alpha coactivator antibody
      • PGC1 antibody
      • PPAR gamma coactivator-1beta antibody
      • PPAR-gamma coactivator 1-beta antibody
      • PPARGC-1-beta antibody
      • PPARGC1 antibody
      • Ppargc1b antibody
      • PRGC2_HUMAN antibody
      see all

    Images

    • ab176328 (purified) at 1/20 immunoprecipitating PGC1 beta in HeLa whole cell lysate.

      Lane 1 (input): HeLa whole cell lysate (10µg)

      Lane 2 (+): ab176328 + HeLa whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab176328 in HeLa whole cell lysate.

      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176328).

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling PGC1 beta using unpurified ab176328 at a dilution of 1/10.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176328).

    References

    ab240188 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab240188.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up