Overview

  • Product name
  • Description
    Rabbit polyclonal to PGD
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Pig
  • Immunogen

    Synthetic peptide within Human PGD aa 242-271 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. NP_002622.2.
    Database link: P52209

  • Positive control
    • MCF7 cell lysate, Mouse spleen tissue lysate, Human lung carcinoma tissue, 293 cells and 293 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab175116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 53 kDa.

Target

Images

  • All lanes : Anti-PGD antibody (ab175116) at 1/1000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : MCF7 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 53 kDa

  • Anti-PGD antibody (ab175116) at 1/1000 dilution + Mouse spleen tissue lysate at 35 µg

    Predicted band size: 53 kDa

  • Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram) labeling PGD with ab175116 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemistry analysis of formalin fixed and paraffin embedded Human lung carcinoma tissue labeling PGD with ab175116 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab175116 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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