Overview

  • Product name

    Anti-PGD antibody [EPR6565]
    See all PGD primary antibodies
  • Description

    Rabbit monoclonal [EPR6565] to PGD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PGD aa 400-500. The exact sequence is proprietary.

  • Positive control

    • WB: Raji, Molt-4, and 293T cell lysates. ICC/IF: HeLa cells. Flow Cyt: HEK293 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 53 kDa).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    Images

    • All lanes : Anti-PGD antibody [EPR6565] (ab129199) at 1/5000 dilution

      Lane 1 : Mouse brain tissue lysates
      Lane 2 : Mouse heart tissue lysates
      Lane 3 : Mouse kidney tissue lysates
      Lane 4 : Mouse spleen tissue lysates
      Lane 5 : Rat brain tissue lysates
      Lane 6 : Rat heart tissue lysates
      Lane 7 : Rat kidney tissue lysates
      Lane 8 : Rat spleen tissue lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 53 kDa
      Observed band size: 45 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PGD with ab129199 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
      Control: PBS only.
      Nuclear counter stain: DAPI.

    • Overlay histogram showing HEK293 cells stained with ab129199 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129199, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • All lanes : Anti-PGD antibody [EPR6565] (ab129199) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Molt-4 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 53 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Baardman J  et al. A Defective Pentose Phosphate Pathway Reduces Inflammatory Macrophage Responses during Hypercholesterolemia. Cell Rep 25:2044-2052.e5 (2018). Read more (PubMed: 30463003) »
    • Yoshida K  et al. Effect of everolimus on the glucose metabolic pathway in mouse skeletal muscle cells (C2C12). Metabolomics 13:98 (2017). Read more (PubMed: 28781589) »
    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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