PGF2 alpha ELISA Kit (ab133041)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
PGF2 alpha ELISA Kit
See all PGF2 alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Low 83pg/ml 13.1% Medium 405pg/ml 6.8% High 916pg/ml 4.9% Inter-assay Sample n Mean SD CV% Low 47pg/ml 9.7% Medium 290pg/ml 5.5% High 731pg/ml 3.1% -
Sample type
Saliva, Urine, Serum, Plasma -
Assay type
Competitive -
Sensitivity
6.71 pg/ml -
Range
3.05 pg/ml - 50000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 103.5 % - % Urine 107 % - % Serum 100.3 % - % Plasma 96.9 % - % -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human, Pig, a wide range of other species -
Product overview
Abcam’s PGF2 alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of PGF2 alpha in Porcine Plasma, Porcine and Human Serum, Saliva, Urine and Tissue Culture Media.
A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-PGF2 alpha antigen and a polyclonal sheep antibody specific to PGF2 alpha. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of PGF2 alpha captured in the plate.
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Notes
Prostaglandin F2α (PGF2α) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF2α is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2α have also been associated with pre-eclampsia.
Cross Reactivity
Compound % Cross Reactivity PGF2α 100 PGF1α 11.82 PGD2 3.62 6-keto-PGF1α 1.38 PGI2 1.25 PGE2 0.77 Thromboxane B2 0.77 8-iso PGF2α 0.73 PGE1 0.39 PGA2 <0.10 6,15-keto-13,14-dihydro-PGF1α <0.01 2-Arachidonoylglycerol <0.01 Anandamide <0.01 -
Tested applications
Suitable for: Competitive ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 30ml Assay Buffer 1 x 30ml Direct 8-iso-PGF2 alpha Antibody 1 x 5ml Donkey anti-sheep IgG Microplate (12 x 8 wells) 1 unit PGF2α Standard 1 x 0.5ml PGF2 alpha Alkaline Phosphatase Conjugate 1 x 5ml Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
Research areas
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Alternative names
- Prostaglandin F2 alpha
Applications
Our Abpromise guarantee covers the use of ab133041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Competitive ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative Standard Curve using ab133041.
Protocols
References
This product has been referenced in:
- Lai CY et al. Physicochemistry and cardiovascular toxicity of metal fume PM2.5: a study of human coronary artery endothelial cells and welding workers. Sci Rep 6:33515 (2016). Human . Read more (PubMed: 27641436) »
- Shin D et al. Dietary Compound Kaempferol Inhibits Airway Thickening Induced by Allergic Reaction in a Bovine Serum Albumin-Induced Model of Asthma. Int J Mol Sci 16:29980-95 (2015). Read more (PubMed: 26694364) »
