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PGF2 alpha ELISA Kit (ab133041)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve

    Key features and details

    • Sensitivity: 6.71 pg/ml
    • Range: 3.05 pg/ml - 50000 pg/ml
    • Sample type: Plasma, Saliva, Serum, Urine
    • Detection method: Colorimetric
    • Assay type: Competitive
    • Reacts with: Human, Pig, a wide range of other species

    Overview

    • Product name

      PGF2 alpha ELISA Kit
      See all PGF2 alpha kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Low 83pg/ml 13.1%
      Medium 405pg/ml 6.8%
      High 916pg/ml 4.9%
      Inter-assay
      Sample n Mean SD CV%
      Low 47pg/ml 9.7%
      Medium 290pg/ml 5.5%
      High 731pg/ml 3.1%
    • Sample type

      Saliva, Urine, Serum, Plasma
    • Assay type

      Competitive
    • Sensitivity

      6.71 pg/ml
    • Range

      3.05 pg/ml - 50000 pg/ml
    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Saliva 103.5 % - %
      Urine 107 % - %
      Serum 100.3 % - %
      Plasma 96.9 % - %
    • Assay time

      3h 00m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human, Pig, a wide range of other species
    • Product overview

      Abcam’s PGF2 alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of PGF2 alpha in Porcine Plasma, Porcine and Human Serum, Saliva, Urine and Tissue Culture Media.

      A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-PGF2 alpha antigen and a polyclonal sheep antibody specific to PGF2 alpha. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of PGF2 alpha captured in the plate.

    • Notes

      Prostaglandin F2α (PGF2α) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF2α is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2α have also been associated with pre-eclampsia.

      Cross Reactivity

      Compound% Cross Reactivity
      PGF2α100
      PGF1α11.82
      PGD23.62
      6-keto-PGF1α1.38
      PGI21.25
      PGE20.77
      Thromboxane B20.77
      8-iso PGF2α0.73
      PGE10.39
      PGA2<0.10
      6,15-keto-13,14-dihydro-PGF1α<0.01
      2-Arachidonoylglycerol<0.01
      Anandamide<0.01
    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Wash Buffer Concentrate 1 x 30ml
      Assay Buffer 1 x 30ml
      Direct 8-iso-PGF2 alpha Antibody 1 x 5ml
      Donkey anti-sheep IgG Microplate (12 x 8 wells) 1 unit
      PGF2α Standard 1 x 0.5ml
      PGF2 alpha Alkaline Phosphatase Conjugate 1 x 5ml
      Plate Sealer 1 unit
      pNpp Substrate 1 x 20ml
      Stop Solution 1 x 5ml
    • Research areas

      • Cell Biology
      • Other Antibodies
      • Drugs and Chemicals
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Eicosanoid ELISA Kits
    • Alternative names

      • Prostaglandin F2 alpha

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab133041.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (3)

    Publishing research using ab133041? Please let us know so that we can cite the reference in this datasheet.

    ab133041 has been referenced in 3 publications.

    • Ahmad AA  et al. Sex differences in the role of phospholipase A2 -dependent arachidonic acid pathway in the perivascular adipose tissue function in pigs. J Physiol 595:6623-6634 (2017). PubMed: 28877347
    • Lai CY  et al. Physicochemistry and cardiovascular toxicity of metal fume PM2.5: a study of human coronary artery endothelial cells and welding workers. Sci Rep 6:33515 (2016). Human . PubMed: 27641436
    • Shin D  et al. Dietary Compound Kaempferol Inhibits Airway Thickening Induced by Allergic Reaction in a Bovine Serum Albumin-Induced Model of Asthma. Int J Mol Sci 16:29980-95 (2015). PubMed: 26694364

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