Overview

  • Product name

    PGF2 alpha High Sensitivity ELISA Kit
    See all PGF2 alpha kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    107.5pmol/ml 24 6.53%
    33.79pmol/ml 24 4.94%
    11.5pmol/ml 24 7.16%
    Inter-assay
    Sample n Mean SD CV%
    117.8pmol/ml 10.97%
    35.81pmol/ml 8.88%
    10.1pmol/ml 10.14%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay type

    Competitive
  • Sensitivity

    0.98 pg/ml
  • Range

    1.95 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 103.5 % - %
    Urine 107 % - %
    Serum 100.3 % - %
    Plasma 96.9 % - %

  • Assay time

    2h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity


    Predicted to work with: a wide range of other species
  • Product overview

    Abcam’s PGF High Sensitivity in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of PGF in milk, culture supernatants, saliva, urine, serum and plasma.

    A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-PGF antigen and a polyclonal sheep antibody specific to PGF. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of PGF captured in the plate.

  • Notes

    Prostaglandin F (PGF) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF have also been associated with preeclampsia.

     

    CompoundCross Reactivity
    PGF 100%
    PGF 11.82%
    PGD2 3.62%
    6-keto-PGF 1.38%
    PGI2 1.25%
    PGE2 0.77%
    Thromboxane B2 0.77%
    8-iso PGF 0.73%
    PGE1 0.39%
    PGA2 <0.1%
    6, 15-keto-13, 14-dihydro-PGF <0.01%
    2-Arachidonoylglycerol <0.01%
    Anandamide <0.01%
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133056.

Protocols

References

This product has been referenced in:

  • Díaz-Viraqué F  et al. Old Yellow Enzyme from Trypanosoma cruzi Exhibits In Vivo Prostaglandin F2a Synthase Activity and Has a Key Role in Parasite Infection and Drug Susceptibility. Front Immunol 9:456 (2018). Read more (PubMed: 29563916) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

I am sorry for the confusion.

I meant the sample which have low levels of PGF2a. Your statement is indeed correct.

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Answer

Thank you for contacting us.

The kit is suitable for both plasma and serum.

The extraction is not mandatory; it is advised for samples with low level PGF2a.

The extracted sample can be stored for months at -80C.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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