Anti-PGGT1B antibody (ab122122)
Key features and details
- Rabbit polyclonal to PGGT1B
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-PGGT1B antibody -
Description
Rabbit polyclonal to PGGT1B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
antigen sequence, corresponding to amino acids 213-347 of Human PGGT1B.
-
Positive control
- IHC: Human duodenum, Fallopian tube, endometrium, testis and pancreas tissues. WB: Human small intestine tissue lysate; Mouse NIH/3T3 cell lysate; Rat NBT-II cell lysate. ICC/IF: Human cell line MCF7.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab122122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. | |
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.04 - 0.4 µg/ml. |
Target
-
Function
Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. Acts on the Rac1, Rac2, Rap1A and Rap1B proteins. The beta subunit is responsible for peptide-binding. -
Sequence similarities
Belongs to the protein prenyltransferase subunit beta family.
Contains 4 PFTB repeats. - Information by UniProt
-
Database links
- Entrez Gene: 5229 Human
- Entrez Gene: 225467 Mouse
- Entrez Gene: 81746 Rat
- Omim: 602031 Human
- SwissProt: P53609 Human
- SwissProt: Q8BUY9 Mouse
- SwissProt: P53610 Rat
- Unigene: 254006 Human
see all -
Alternative names
- BGGI antibody
- Geranylgeranyl transferase type I subunit beta antibody
- Geranylgeranyl transferase type-1 subunit beta antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGGT1B antibody (ab122122)
Immunohistochemical analysis of human testis tissue labeling PGGT1B with ab122122 at a 1/200 dilution.
-
Western blot - Anti-PGGT1B antibody (ab122122)Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) -
Immunocytochemistry/ Immunofluorescence - Anti-PGGT1B antibody (ab122122)Immunocytochemsitry of MCF7 (Human breast adenocarcinoma cell line) cells labeling PGGT1B with ab122122 at a 0.25 ug/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGGT1B antibody (ab122122)Immunohistochemical analysis of human duodenum tissue labeling PGGT1B with ab122122 at a 1/200 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGGT1B antibody (ab122122)Immunohistochemical analysis of human Fallopian tube tissue labeling PGGT1B with ab122122 at a 1/200 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGGT1B antibody (ab122122)Immunohistochemical analysis of human endometrium tissue labeling PGGT1B with ab122122 at a 1/200 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGGT1B antibody (ab122122)Immunohistochemical analysis of human pancreas tissue labeling PGGT1B with ab122122 at a 1/200 dilution.
-
Western blot - Anti-PGGT1B antibody (ab122122)Anti-PGGT1B antibody (ab122122) at 0.04 µg/ml + Human small intestine tissue lysateWestern blot analysis of human small intestine tissue lysate labeling PGGT1B with ab122122 at 0.04 ug/ml.
Protocols
Datasheets and documents
References (2)
ab122122 has been referenced in 2 publications.
- Ye D et al. MicroRNA-125a-mediated regulation of the mevalonate signaling pathway contributes to high glucose-induced proliferation and migration of vascular smooth muscle cells. Mol Med Rep 22:165-174 (2020). PubMed: 32319638
- Chen B et al. Alteration of mevalonate pathway related enzyme expressions in pressure overload-induced cardiac hypertrophy and associated heart failure with preserved ejection fraction. Cell Physiol Biochem 32:1761-75 (2013). PubMed: 24356578
