Anti-PGGT1B antibody (ab122122)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PGGT1B antibody -
Description
Rabbit polyclonal to PGGT1B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
antigen sequence, corresponding to amino acids 213-347 of Human PGGT1B.
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Positive control
- Human breast tissue; RT 4, U 251 MG, Human liver and tonsil lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab122122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/250 - 1/500. |
Target
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Function
Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. Acts on the Rac1, Rac2, Rap1A and Rap1B proteins. The beta subunit is responsible for peptide-binding. -
Sequence similarities
Belongs to the protein prenyltransferase subunit beta family.
Contains 4 PFTB repeats. - Information by UniProt
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Database links
- Entrez Gene: 5229 Human
- Entrez Gene: 225467 Mouse
- Entrez Gene: 81746 Rat
- Omim: 602031 Human
- SwissProt: P53609 Human
- SwissProt: Q8BUY9 Mouse
- SwissProt: P53610 Rat
- Unigene: 254006 Human
see all -
Alternative names
- BGGI antibody
- Geranylgeranyl transferase type I subunit beta antibody
- Geranylgeranyl transferase type-1 subunit beta antibody
see all
Images
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Western blot - Anti-PGGT1B antibody (ab122122)Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) -
Western blot - Anti-PGGT1B antibody (ab122122)All lanes : Anti-PGGT1B antibody (ab122122) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysateDeveloped using the ECL technique
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGGT1B antibody (ab122122)ab122122, at a 1/75 dilution, staining PGGT1B in paraffin-embedded Human breast tissue by Immunohistochemistry.
Datasheets and documents
References
ab122122 has not yet been referenced specifically in any publications.
