Key features and details
- Rabbit polyclonal to PGGT1B
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-PGGT1B antibody
DescriptionRabbit polyclonal to PGGT1B
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
antigen sequence, corresponding to amino acids 213-347 of Human PGGT1B.
- IHC: Human duodenum, Fallopian tube, endometrium, testis and pancreas tissues. WB: Human small intestine tissue lysate; Mouse NIH/3T3 cell lysate; Rat NBT-II cell lysate. ICC/IF: Human cell line MCF7.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionCatalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. Acts on the Rac1, Rac2, Rap1A and Rap1B proteins. The beta subunit is responsible for peptide-binding.
Sequence similaritiesBelongs to the protein prenyltransferase subunit beta family.
Contains 4 PFTB repeats.
- Information by UniProt
- BGGI antibody
- Geranylgeranyl transferase type I subunit beta antibody
- Geranylgeranyl transferase type-1 subunit beta antibody
Immunohistochemical analysis of human testis tissue labeling PGGT1B with ab122122 at a 1/200 dilution.
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemsitry of MCF7 (Human breast adenocarcinoma cell line) cells labeling PGGT1B with ab122122 at a 0.25 ug/ml.
Immunohistochemical analysis of human duodenum tissue labeling PGGT1B with ab122122 at a 1/200 dilution.
Immunohistochemical analysis of human Fallopian tube tissue labeling PGGT1B with ab122122 at a 1/200 dilution.
Immunohistochemical analysis of human endometrium tissue labeling PGGT1B with ab122122 at a 1/200 dilution.
Immunohistochemical analysis of human pancreas tissue labeling PGGT1B with ab122122 at a 1/200 dilution.
Anti-PGGT1B antibody (ab122122) at 0.04 µg/ml + Human small intestine tissue lysate
Western blot analysis of human small intestine tissue lysate labeling PGGT1B with ab122122 at 0.04 ug/ml.
ab122122 has been referenced in 2 publications.
- Ye D et al. MicroRNA-125a-mediated regulation of the mevalonate signaling pathway contributes to high glucose-induced proliferation and migration of vascular smooth muscle cells. Mol Med Rep 22:165-174 (2020). PubMed: 32319638
- Chen B et al. Alteration of mevalonate pathway related enzyme expressions in pressure overload-induced cardiac hypertrophy and associated heart failure with preserved ejection fraction. Cell Physiol Biochem 32:1761-75 (2013). PubMed: 24356578