HRP Anti-PGK1 antibody [22C5D8] (ab197960)
Key features and details
- HRP Mouse monoclonal [22C5D8] to PGK1
- Suitable for: WB, IHC-P
- Reacts with: Human, Saccharomyces cerevisiae
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
HRP Anti-PGK1 antibody [22C5D8]
See all PGK1 primary antibodies -
Description
HRP Mouse monoclonal [22C5D8] to PGK1 -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Saccharomyces cerevisiae -
Immunogen
Full length native protein (purified) corresponding to PGK1.
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Positive control
- WB: Saccharomyces cerevisiae (Y190) cell lysate. IHC-P: FFPE human colon tissue sections.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation. -
Clonality
Monoclonal -
Clone number
22C5D8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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IHC-P |
1/70 - 1/80. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
IHC-P
1/70 - 1/80. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. -
Involvement in disease
Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations. -
Sequence similarities
Belongs to the phosphoglycerate kinase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5230 Human
- Entrez Gene: 850370 Saccharomyces cerevisiae
- Omim: 311800 Human
- SwissProt: P00558 Human
- SwissProt: P00560 Saccharomyces cerevisiae
- Unigene: 78771 Human
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Alternative names
- Cell migration-inducing gene 10 protein antibody
- Epididymis secretory sperm binding protein Li 68p antibody
- HEL S 68p antibody
see all
Images
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IHC image of PGK1 staining in a section of formalin-fixed paraffin-embedded human normal colon*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab197960 at 1/71 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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HRP Anti-PGK1 antibody [22C5D8] (ab197960) at 1/5000 dilution + S.cerevisiae (Y190) Whole Cell Lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197960 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab197960 has been referenced in 7 publications.
- Stolp ZD et al. Yeast cell death pathway requiring AP-3 vesicle trafficking leads to vacuole/lysosome membrane permeabilization. Cell Rep 39:110647 (2022). PubMed: 35417721
- Chio US et al. Subunit cooperation in the Get1/2 receptor promotes tail-anchored membrane protein insertion. J Cell Biol 220:N/A (2021). PubMed: 34614151
- Schütter M et al. Local Fatty Acid Channeling into Phospholipid Synthesis Drives Phagophore Expansion during Autophagy. Cell 180:135-149.e14 (2020). PubMed: 31883797
- Chio US et al. A Chaperone Lid Ensures Efficient and Privileged Client Transfer during Tail-Anchored Protein Targeting. Cell Rep 26:37-44.e7 (2019). PubMed: 30605684
- Jenkins SS et al. Role of the Srs2-Rad51 Interaction Domain in Crossover Control in Saccharomyces cerevisiae. Genetics 212:1133-1145 (2019). PubMed: 31142613
- Lynch KL et al. The effects of manipulating levels of replication initiation factors on origin firing efficiency in yeast. PLoS Genet 15:e1008430 (2019). PubMed: 31584938
- Talhaoui I et al. Slx5-Slx8 ubiquitin ligase targets active pools of the Yen1 nuclease to limit crossover formation. Nat Commun 9:5016 (2018). PubMed: 30479332