Overview

  • Product name

    Anti-PGK1 antibody [22C5D8] (HRP)
    See all PGK1 primary antibodies
  • Description

    Mouse monoclonal [22C5D8] to PGK1 (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Full length native protein (purified) corresponding to PGK1.

  • Positive control

    • WB: Saccharomyces cerevisiae (Y190) cell lysate. IHC-P: FFPE human colon tissue sections.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab197960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/70 - 1/80. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
  • Involvement in disease

    Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
  • Sequence similarities

    Belongs to the phosphoglycerate kinase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

    • Alternative names

      • Cell migration-inducing gene 10 protein antibody
      • Epididymis secretory sperm binding protein Li 68p antibody
      • HEL S 68p antibody
      • MGC117307 antibody
      • MGC8947 antibody
      • MIG10 antibody
      • pgk1 antibody
      • PGK1_HUMAN antibody
      • PGKA antibody
      • Phosphoglycerate kinase 1 antibody
      • Primer recognition protein 2 antibody
      • PRP 2 antibody
      see all

    Images

    • IHC image of PGK1 staining in a section of formalin-fixed paraffin-embedded human normal colon*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab197960 at 1/71 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Anti-PGK1 antibody [22C5D8] (HRP) (ab197960) at 1/5000 dilution + S.cerevisiae (Y190) Whole Cell Lysate at 20 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 45 kDa
      Observed band size: 45 kDa


      Exposure time: 1 minute


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197960 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    References

    This product has been referenced in:

    • Chio US  et al. A Chaperone Lid Ensures Efficient and Privileged Client Transfer during Tail-Anchored Protein Targeting. Cell Rep 26:37-44.e7 (2019). Read more (PubMed: 30605684) »
    • Talhaoui I  et al. Slx5-Slx8 ubiquitin ligase targets active pools of the Yen1 nuclease to limit crossover formation. Nat Commun 9:5016 (2018). Read more (PubMed: 30479332) »
    See all 2 Publications for this product

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