Overview

  • Product name

  • Description

    Rabbit polyclonal to PGK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae
  • Immunogen

    A recombinant fragment corresponding to a region within amino acids 1-217 of Human PGK1 (Uniprot ID P00558).

  • Positive control

    • A549, HeLa, mouse brain and rat liver whole cell lysates. Yeast wild type 303. HeLa cells. Human hepatoma tissue.
  • General notes

    Store as a concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
  • Involvement in disease

    Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
  • Sequence similarities

    Belongs to the phosphoglycerate kinase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell migration-inducing gene 10 protein antibody
    • Epididymis secretory sperm binding protein Li 68p antibody
    • HEL S 68p antibody
    • MGC117307 antibody
    • MGC8947 antibody
    • MIG10 antibody
    • pgk1 antibody
    • PGK1_HUMAN antibody
    • PGKA antibody
    • Phosphoglycerate kinase 1 antibody
    • Primer recognition protein 2 antibody
    • PRP 2 antibody
    see all

Images

  • All lanes : Anti-PGK1 antibody (ab154613) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Anti-PGK1 antibody (ab154613) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 45 kDa



    10% SDS PAGE
  • All lanes : Anti-PGK1 antibody (ab154613) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate (untreated)
    Lane 2 : HeLa whole cell lysate (1% O2 treatment for 24hr)

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Anti-PGK1 antibody (ab154613) at 1/1000 dilution + Rat liver whole cell lysate at 50 µg

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Hepatoma, labeling PGK1 using ab154613 at 1/500 dilution.
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling PGK1 using ab154613 at 1/50 dilution. Right panel costained with Hoechst 33342.

References

ab154613 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (B16F10)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
20 µg
Specification
B16F10
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 11 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (WM266-human melanoma)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
20 µg
Specification
WM266-human melanoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 10 2018

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