Anti-PGK1 antibody (ab154613)
Key features and details
- Rabbit polyclonal to PGK1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PGK1 antibody
See all PGK1 primary antibodies -
Description
Rabbit polyclonal to PGK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae -
Immunogen
A recombinant fragment corresponding to a region within amino acids 1-217 of Human PGK1 (Uniprot ID P00558).
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Positive control
- A549, HeLa, mouse brain and rat liver whole cell lysates. Yeast wild type 303. HeLa cells. Human hepatoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. -
Involvement in disease
Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations. -
Sequence similarities
Belongs to the phosphoglycerate kinase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5230 Human
- Entrez Gene: 18655 Mouse
- Entrez Gene: 24644 Rat
- Entrez Gene: 850370 Saccharomyces cerevisiae
- Omim: 311800 Human
- SwissProt: P00558 Human
- SwissProt: P09411 Mouse
- SwissProt: P16617 Rat
see all -
Alternative names
- Cell migration-inducing gene 10 protein antibody
- Epididymis secretory sperm binding protein Li 68p antibody
- HEL S 68p antibody
see all
Images
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All lanes : Anti-PGK1 antibody (ab154613) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE -
Anti-PGK1 antibody (ab154613) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 45 kDa
10% SDS PAGE -
All lanes : Anti-PGK1 antibody (ab154613) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate (untreated)
Lane 2 : HeLa whole cell lysate (1% O2 treatment for 24hr)
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE -
Anti-PGK1 antibody (ab154613) at 1/1000 dilution + Rat liver whole cell lysate at 50 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded human Hepatoma, labeling PGK1 using ab154613 at 1/500 dilution.
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Immunofluorescence analysis of methanol-fixed HeLa cells, labeling PGK1 using ab154613 at 1/50 dilution. Right panel costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154613 has been referenced in 2 publications.
- Ortega MA et al. Overexpression of glycolysis markers in placental tissue of pregnant women with chronic venous disease: a histological study. Int J Med Sci 19:186-194 (2022). PubMed: 34975312
- Fraile-Martinez O et al. Venous Wall of Patients with Chronic Venous Disease Exhibits a Glycolytic Phenotype. J Pers Med 12:N/A (2022). PubMed: 36294781