Anti-PGK1 antibody (ab233135)
Key features and details
- Rabbit polyclonal to PGK1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PGK1 antibody
See all PGK1 primary antibodies -
Description
Rabbit polyclonal to PGK1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Pig, Chimpanzee, Cynomolgus monkey, Chinese hamster, Orangutan -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human PGK1 aa 1 to the C-terminus. Two N-terminal tags. Expressed in E.coli.
Database link: P00558 -
Positive control
- WB: Recombinant human PGK1 protein; HeLa, HepG2 and HEK-293T cell lysates. IHC-P: Human cerebrum, colorectal cancer, glioma, liver and kidney tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 45 kDa.
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 45 kDa. |
ICC/IF
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. -
Involvement in disease
Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations. -
Sequence similarities
Belongs to the phosphoglycerate kinase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5230 Human
- Entrez Gene: 18655 Mouse
- Entrez Gene: 24644 Rat
- Omim: 311800 Human
- SwissProt: A5A6K4 Chimpanzee
- SwissProt: P50310 Chinese hamster
- SwissProt: Q3T0P6 Cow
- SwissProt: P00559 Horse
see all -
Alternative names
- Cell migration-inducing gene 10 protein antibody
- Epididymis secretory sperm binding protein Li 68p antibody
- HEL S 68p antibody
see all
Images
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Anti-PGK1 antibody (ab233135) at 2 µg/ml + Recombinant human PGK1 protein
Predicted band size: 45 kDa -
All lanes : Anti-PGK1 antibody (ab233135) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 45 kDa -
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for PGK1 using ab233135 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for PGK1 using ab233135 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human glioma tissue stained for PGK1 using ab233135 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for PGK1 using ab233135 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for PGK1 using ab233135 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PGK1 (green) using ab233135 at 20 μg/ml in ICC/IF. FITC labeling.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab233135 has been referenced in 1 publication.
- Liang J et al. LncRNA NEAT1 facilitates glioma progression via stabilizing PGK1. J Transl Med 20:80 (2022). PubMed: 35123484