Product nameAnti-PGK1 antibody
See all PGK1 primary antibodies
DescriptionRabbit polyclonal to PGK1
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ELISAmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Saccharomyces cerevisiae
Synthetic peptide conjugated to KLH selected within aa 100~200 of human PGK1.
- HepG2 cell lysate. Mouse stomach tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody is purified through a protein G column, eluted out with both high and low pH buffers, neutralized immediately after elution then followed by dialysis against PBS.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab38007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 44 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionIn addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Involvement in diseaseDefects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
Sequence similaritiesBelongs to the phosphoglycerate kinase family.
- Information by UniProt
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All lanes : Anti-PGK1 antibody (ab38007) at 1/100 dilution
Lane 1 : Mouse stomach tissue lysate
Lane 2 : HepG2 cell lysate
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
ab38007 at 1/100 dilution staining PGK1 in human Hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
ICC/IF image of ab38007 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38007, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Suresh SN et al. Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRa Is Neuroprotective. Front Mol Neurosci 11:109 (2018). Read more (PubMed: 29686608) »
- Suresh SN et al. A novel autophagy modulator 6-Bio ameliorates SNCA/a-synuclein toxicity. Autophagy 13:1221-1234 (2017). Read more (PubMed: 28350199) »