Western blot abreview for Anti-PGK1 antibody

Poor
Abreviews
Application
Western blot
Sample
Schizosaccharomyces pombe Cell lysate - whole cell (Whole cell lysate)
Loading amount
100 µg
Specification
Whole cell lysate
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: BD, anti-rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/2000

Detection

Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: n/a kDa Non-specific: 35, 38, 49, 60 kDa
Positive control
S. cerevisiae TCA whole cell lysate.
Negative control
n/a

Additional data

Abcam response
Thank you for your valuable feedback. S. pombe is not a tested species for ab38007 and it seems that it is still unclear how specific this antibody is against S. pombe PGK1 under your experimental conditions. However, it appears that this antibody does recognize PGK1 in S. cerevisiae. Thank you for your time - we hope that this information will be helpful for future customers.

Abcam user community

Verified customer

Submitted Aug 02 2010

Sign up