Recombinant Anti-PGK2 antibody [EPR14909(B)] (ab183031)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14909(B)] to PGK2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PGK2 antibody [EPR14909(B)]
See all PGK2 primary antibodies -
Description
Rabbit monoclonal [EPR14909(B)] to PGK2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human, mouse and rat testis and Human seminoma lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14909(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa). |
Target
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Tissue specificity
Testis specific. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. -
Sequence similarities
Belongs to the phosphoglycerate kinase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5232 Human
- Entrez Gene: 18663 Mouse
- Entrez Gene: 316265 Rat
- Omim: 172270 Human
- SwissProt: P07205 Human
- SwissProt: P09041 Mouse
- Unigene: 367727 Human
- Unigene: 717 Mouse
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Alternative names
- dJ417L20.2 antibody
- PGK 2 antibody
- pgk2 antibody
see all
Images
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All lanes : Anti-PGK2 antibody [EPR14909(B)] (ab183031) at 1/1000 dilution
Lane 1 : PGK1 Human Recombinant Protein
Lane 2 : PGK2 Human Recombinant Protein
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Exposure time: 10 secondsBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-PGK2 antibody [EPR14909(B)] (ab183031) at 1/1000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 45 kDa -
All lanes : Anti-PGK2 antibody [EPR14909(B)] (ab183031) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Human seminoma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 45 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab183031 has been referenced in 4 publications.
- Liu X et al. Identification of abnormal protein expressions associated with mouse spermatogenesis induced by cyclophosphamide. J Cell Mol Med 25:1624-1632 (2021). PubMed: 33438283
- Chen W et al. The Neurogenic Compound P7C3 Regulates the Aerobic Glycolysis by Targeting Phosphoglycerate Kinase 1 in Glioma. Front Oncol 11:644492 (2021). PubMed: 34221965
- Wu ST et al. Esculetin Inhibits Cancer Cell Glycolysis by Binding Tumor PGK2, GPD2, and GPI. Front Pharmacol 11:379 (2020). PubMed: 32292350
- Liu X et al. Aberrant expression of sperm-specific glycolytic enzymes are associated with poor sperm quality. Mol Med Rep 19:2471-2478 (2019). PubMed: 30720094