Key features and details
- Rabbit polyclonal to PGLS
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PGLS antibody
See all PGLS primary antibodies
DescriptionRabbit polyclonal to PGLS
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Recombinant full length protein corresponding to Human PGLS aa 1-258.
MAAPAPGLISVFSSSQELGAALAQLVAQRAACCLAGARARFALGLSGGSL VSMLARELPAAVAPAGPASLARWTLGFCDERLVPFDHAESTYGLYRTHLL SRLPIPESQVITINPELPVEEAAEDYAKKLRQAFQGDSIPVFDLLILGVG PDGHTCSLFPDHPLLQEREKIVAPISDSPKPPPQRVTLTLPVLNAARTVI FVATGEGKAAVLKRILEDQEENPLPAALVQPHTGKLCWFLDEAAARLLTV PFEKHSTL
Database link: O95336
- WB: Mouse liver and brain lysates; U-251 MG, THP-1, HepG2 and HeLa whole cell lysates. IHC-P: Human thyroid and tonsil tissues. IP: U-251 MG whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 28 kDa.|
|IP||1/200 - 1/2000.|
RelevancePGLS belongs to the glucosamine/galactosamine-6-phosphate isomerase family, 6-phosphogluconolactonase subfamily. It is implicated in the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.
- 6 phosphogluconolactonase antibody
- 6PGL antibody
All lanes : Anti-PGLS antibody (ab229980) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse brain lysate
Lane 3 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28,52 kDa why is the actual band size different from the predicted?
Paraffin-embedded human thyroid tissue stained for PGLS using ab229980 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human tonsil tissue stained for PGLS using ab229980 at 1/100 dilution in immunohistochemical analysis.
PGLS was immunoprecipitated from 500 µg U-251 MG (human brain glioma cell line) whole cell lysate with 4 µg ab229980.
Lane 1: Rabbit monoclonal IgG (1 µg) IP in U-251 MG whole cell lysate.
Lane 2: ab229980 IP in U-251 MG whole cell lysate.
Lane 3: U-251 MG whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229980 has not yet been referenced specifically in any publications.