Product nameAnti-PGLS antibody
See all PGLS primary antibodies
DescriptionRabbit polyclonal to PGLS
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Recombinant full length protein corresponding to Human PGLS aa 1-258.
MAAPAPGLISVFSSSQELGAALAQLVAQRAACCLAGARARFALGLSGGSL VSMLARELPAAVAPAGPASLARWTLGFCDERLVPFDHAESTYGLYRTHLL SRLPIPESQVITINPELPVEEAAEDYAKKLRQAFQGDSIPVFDLLILGVG PDGHTCSLFPDHPLLQEREKIVAPISDSPKPPPQRVTLTLPVLNAARTVI FVATGEGKAAVLKRILEDQEENPLPAALVQPHTGKLCWFLDEAAARLLTV PFEKHSTL
Database link: O95336
- WB: Mouse liver and brain lysates; U-251 MG, THP-1, HepG2 and HeLa whole cell lysates. IHC-P: Human thyroid and tonsil tissues. IP: U-251 MG whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- Mouse liver tissue lysate - total protein (ab29301)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (ab4022)
- HepG2 whole cell lysate (ab7900)
- THP1 whole cell lysate (ab7913)
Our Abpromise guarantee covers the use of ab229980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 28 kDa.|
|IP||1/200 - 1/2000.|
RelevancePGLS belongs to the glucosamine/galactosamine-6-phosphate isomerase family, 6-phosphogluconolactonase subfamily. It is implicated in the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.
- 6 phosphogluconolactonase antibody
- 6PGL antibody
All lanes : Anti-PGLS antibody (ab229980) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse brain lysate
Lane 3 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28,52 kDa why is the actual band size different from the predicted?
Paraffin-embedded human thyroid tissue stained for PGLS using ab229980 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human tonsil tissue stained for PGLS using ab229980 at 1/100 dilution in immunohistochemical analysis.
PGLS was immunoprecipitated from 500 µg U-251 MG (human brain glioma cell line) whole cell lysate with 4 µg ab229980.
Lane 1: Rabbit monoclonal IgG (1 µg) IP in U-251 MG whole cell lysate.
Lane 2: ab229980 IP in U-251 MG whole cell lysate.
Lane 3: U-251 MG whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
ab229980 has not yet been referenced specifically in any publications.