Key features and details
- Rabbit polyclonal to PGM1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PGM1 antibody
See all PGM1 primary antibodies
DescriptionRabbit polyclonal to PGM1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human PGM1 aa 1-562.
Database link: P36871
- WB: U-251 MG, U937, Jurkat and HeLa whole cell lysates. IHC-P: Human liver cancer and testis tissue. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%
Our Abpromise guarantee covers the use of ab237523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 61 kDa.|
|IHC-P||1/20 - 1/200.|
FunctionThis enzyme participates in both the breakdown and synthesis of glucose.
Involvement in diseaseDefects in PGM1 are the cause of glycogen storage disease type 14 (GSD14) [MIM:612934]. A metabolic disorder resulting in a myopathy characterized by exercise-induced intolerance with episodes of rhabdomyolysis, normal elevation of lactate, and hyperammonemia on a forearm-exercise test.
Sequence similaritiesBelongs to the phosphohexose mutase family.
- Information by UniProt
- CDG1T antibody
- Glucose phosphomutase 1 antibody
- GSD14 antibody
All lanes : Anti-PGM1 antibody (ab237523) at 1/500 dilution
Lane 1 : U-251 MG (Human brain glioma cell line) whole cell lysate
Lane 2 : U937 (Human histiocytic lymphoma cell line) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 61 kDa
Paraffin-embedded human liver cancer tissue stained for PGM1 using ab237523 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for PGM1 using ab237523 at 1/100 dilution in immunohistochemical analysis.
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for PGM1 (green) using ab237523 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237523 has not yet been referenced specifically in any publications.