Recombinant Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
![Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)](https://www.abcam.com/ps/products/250/ab250979/Images/ab250979-410538-anti-pgm1-antibody-epr15240-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15240] to PGM1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PGM1 antibody [EPR15240] - BSA and Azide free
See all PGM1 primary antibodies -
Description
Rabbit monoclonal [EPR15240] to PGM1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250979 is the carrier-free version of ab188869.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR15240 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
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| ICC |
Use at an assay dependent concentration.
|
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
|
|
| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
|
IP
Use at an assay dependent concentration. |
Target
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Function
This enzyme participates in both the breakdown and synthesis of glucose. -
Involvement in disease
Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14) [MIM:612934]. A metabolic disorder resulting in a myopathy characterized by exercise-induced intolerance with episodes of rhabdomyolysis, normal elevation of lactate, and hyperammonemia on a forearm-exercise test. -
Sequence similarities
Belongs to the phosphohexose mutase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5236 Human
- Omim: 171900 Human
- SwissProt: P36871 Human
- Unigene: 1869 Human
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Alternative names
- CDG1T antibody
- Glucose phosphomutase 1 antibody
- GSD14 antibody
see all
Images
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Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)All lanes : Anti-PGM1 antibody [EPR15240] (ab188869) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 61 kDaThis data was developed using ab188869, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)](https://www.abcam.com/ps/products/250/ab250979/Images/ab250979-4-anti-pgm1-antibody-epr15240-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)This data was developed using ab188869, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab188869 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Flow Cytometry (Intracellular) - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)](https://www.abcam.com/ps/products/250/ab250979/Images/ab250979-3-anti-pgm1-antibody-epr15240-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)This data was developed using ab188869, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 293T cells (paraformaldehyde-fixed, 2%) labeling PGM1 with ab188869 at 1/360 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Immunocytochemistry - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)](https://www.abcam.com/ps/products/250/ab250979/Images/ab250979-2-anti-pgm1-antibody-epr15240-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)This data was developed using ab188869, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293T cells (4% Paraformaldehyde-fixed) labeling PGM1 with ab188869 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue). -
![Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)](https://www.abcam.com/ps/products/250/ab250979/Images/ab250979-398990-anti-pgm1-antibody-epr15240-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)All lanes : Anti-PGM1 antibody [EPR15240] (ab188869) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal liver lysate
Lane 3 : 293T lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution
Predicted band size: 61 kDaThis data was developed using ab188869, the same antibody clone in a different buffer formulation.
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![Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)](https://www.abcam.com/ps/products/250/ab250979/Images/ab250979-6-benefits-of-recombinant-antibodies.png)
Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250979 has not yet been referenced specifically in any publications.
