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RecombinantRabMAb

Recombinant Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
  • Immunoprecipitation - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
  • Flow Cytometry - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
  • Immunocytochemistry - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
  • Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
  • Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15240] to PGM1 - BSA and Azide free
  • Suitable for: ICC, WB, IP, Flow Cyt
  • Reacts with: Human

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PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)

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Overview

  • Product name

    Anti-PGM1 antibody [EPR15240] - BSA and Azide free
    See all PGM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15240] to PGM1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250979 is the carrier-free version of ab188869. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab250979 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR15240
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-PGM1 antibody [EPR15240] (ab188869)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human PGM1 protein (ab207115)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IP
Human
WB
Human
Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Target

  • Function

    This enzyme participates in both the breakdown and synthesis of glucose.
  • Involvement in disease

    Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14) [MIM:612934]. A metabolic disorder resulting in a myopathy characterized by exercise-induced intolerance with episodes of rhabdomyolysis, normal elevation of lactate, and hyperammonemia on a forearm-exercise test.
  • Sequence similarities

    Belongs to the phosphohexose mutase family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P36871 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5236 Human
    • Omim: 171900 Human
    • SwissProt: P36871 Human
    • Unigene: 1869 Human
    • Alternative names

      • CDG1T antibody
      • Glucose phosphomutase 1 antibody
      • GSD14 antibody
      • OTTHUMP00000010519 antibody
      • OTTHUMP00000010520 antibody
      • PGM 1 antibody
      • PGM1 antibody
      • PGM1_HUMAN antibody
      • Phosphoglucomutase 1 antibody
      • Phosphoglucomutase-1 antibody
      • Phosphoglucomutase1 antibody
      see all

    Images

    • Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
      Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
      All lanes : Anti-PGM1 antibody [EPR15240] (ab188869) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Predicted band size: 61 kDa



      This data was developed using ab188869, the same antibody clone in a different buffer formulation.

    • Immunoprecipitation - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
      Immunoprecipitation - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
      This data was developed using ab188869, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab188869 at 1/50 dilution.
      Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
    • Flow Cytometry - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
      Flow Cytometry - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
      This data was developed using ab188869, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 293T cells (paraformaldehyde-fixed, 2%) labeling PGM1 with ab188869 at 1/360 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
    • Immunocytochemistry - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
      Immunocytochemistry - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
      This data was developed using ab188869, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293T cells (4% Paraformaldehyde-fixed) labeling PGM1 with ab188869 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
    • Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
      Western blot - Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
      All lanes : Anti-PGM1 antibody [EPR15240] (ab188869) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

      Predicted band size: 61 kDa



      This data was developed using ab188869, the same antibody clone in a different buffer formulation.

       

    • Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)
      Anti-PGM1 antibody [EPR15240] - BSA and Azide free (ab250979)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab250979? Please let us know so that we can cite the reference in this datasheet.

    ab250979 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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