Key features and details
- Rabbit polyclonal to PGM3 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PGM3 antibody - C-terminal
See all PGM3 primary antibodies
DescriptionRabbit polyclonal to PGM3 - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T and Jurkat whole cell lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab193007 was affinity purified using an epitope specific to PGM3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab193007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 60 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionInterconverts GlcNAc-6-P and GlcNAc-1-P.
Tissue specificityFound in many tissues except lung. Relatively high expression in pancreas, heart, liver, and placenta, and relatively low expression in brain, skeletal muscle and kidney.
PathwayNucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 2/2.
Sequence similaritiesBelongs to the phosphohexose mutase family.
- Information by UniProt
- 2810473H05Rik antibody
- Acetylglucosamine phosphomutase antibody
- Agm1 antibody
All lanes : Anti-PGM3 antibody - C-terminal (ab193007) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
Detection of PGM3 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab193007 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab193007 has not yet been referenced specifically in any publications.