Overview

  • Product name

    Anti-PGM3 antibody [OTI1A11]
    See all PGM3 primary antibodies
  • Description

    Mouse monoclonal [OTI1A11] to PGM3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human PGM3. Produced in HKE293T cells (NP_056414).
    Database link: O95394

  • Positive control

    • WB: HEK-293T cells transfected with pCMV6-ENTRY PGM3 cDNA; HepG2, HeLa, SVT2, COS-7 and MCF7 cell lysates. IHC-P: Human Kidney, Human liver, Human pancreas, Adenocarcinoma of Human endometrium, Human prostate and Carcinoma of Human prostate tissues. Flow: PGM3 transfected HEK-293T cells
  • General notes

    The clone number has been updated from 1A11 to OTI1A11, both clone numbers name the same antibody clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab128094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 60 kDa.
IHC-P 1/150.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Interconverts GlcNAc-6-P and GlcNAc-1-P.
  • Tissue specificity

    Found in many tissues except lung. Relatively high expression in pancreas, heart, liver, and placenta, and relatively low expression in brain, skeletal muscle and kidney.
  • Pathway

    Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 2/2.
  • Sequence similarities

    Belongs to the phosphohexose mutase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2810473H05Rik antibody
    • Acetylglucosamine phosphomutase antibody
    • Agm1 antibody
    • AGM1_HUMAN antibody
    • BB187688 antibody
    • C77933 antibody
    • DKFZp434B187 antibody
    • EC 5.4.2.3 antibody
    • FLJ11614 antibody
    • FLJ13623 antibody
    • IMD23 antibody
    • N acetylglucosamine phosphate mutase 1 antibody
    • N acetylglucosamine phosphate mutase antibody
    • N-acetylglucosamine-phosphate mutase antibody
    • PAGM antibody
    • PGM 3 antibody
    • PGM3 antibody
    • Phosphoacetylglucosamine mutase antibody
    • Phosphoglucomutase 3 antibody
    • Phosphoglucomutase-3 antibody
    see all

Images

  • All lanes : Anti-PGM3 antibody [OTI1A11] (ab128094) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY PGM3 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 60 kDa

  • All lanes : Anti-PGM3 antibody [OTI1A11] (ab128094) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 (human lung carcinoma cell line) cell lysate
    Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 7 : MDCK cell extract
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
    Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 60 kDa

  • Paraffin-embedded human kidney tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

     

  • Paraffin-embedded human liver tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

     

  • Paraffin-embedded human pancreas tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

     

  • Paraffin-embedded human endometrium adenocarcinoma tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

     

  • Paraffin-embedded human prostate tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

     

  • Paraffin-embedded human prosate carcinoma tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.

     

  • Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) using ab128094 at 1/100 dilution.

     

References

This product has been referenced in:

  • Li J  et al. Correlative study on the JAK-STAT/PSMß3 signal transduction pathway in asthenozoospermia. Exp Ther Med 13:127-130 (2017). Read more (PubMed: 28123480) »
  • Chaveroux C  et al. Nutrient shortage triggers the hexosamine biosynthetic pathway via the GCN2-ATF4 signalling pathway. Sci Rep 6:27278 (2016). WB . Read more (PubMed: 27255611) »
See all 2 Publications for this product

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