Anti-PGP antibody (ab261736)
Key features and details
- Rabbit polyclonal to PGP
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PGP antibody
See all PGP primary antibodies -
Description
Rabbit polyclonal to PGP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PGP aa 250 to the C-terminus.
Database link: A6NDG6 -
Positive control
- WB: PGP overexpression HEK-293T cell lysate. IHC-P: Human testis, cerebral cortex, skeletal muscle and placenta tissue. ICC/IF: MCF7 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab261736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use a concentration of 0.4 µg/ml. Predicted molecular weight: 34 kDa.
Recombinant expression validation in WB using target protein overexpression. |
|
ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
---|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.4 µg/ml. Predicted molecular weight: 34 kDa. Recombinant expression validation in WB using target protein overexpression. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
-
Tissue specificity
Detected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level). The highest activities occur in skeletal muscle and cardiac muscle. -
Sequence similarities
Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family. - Information by UniProt
-
Database links
- Entrez Gene: 283871 Human
- Omim: 172280 Human
- SwissProt: A6NDG6 Human
- Unigene: 442634 Human
-
Alternative names
- PGP antibody
- PGP_HUMAN antibody
- PGPase antibody
- Phosphoglycolate phosphatase antibody
Images
-
Immunohistochemical analysis of human testis tissue labelling PGP with ab261736 shows moderate granular cytoplasmatic positivity in cells in semiferous ducts and Leydig cells.
-
Immunohistochemical analysis of human cerebral cortex tissue labelling PGP with ab261736 shows strong positivity in neuropil.
-
Immunohistochemical analysis of human skeletal muscle tissue labelling PGP with ab261736 shows weak to moderate cytotoplasmatic positivity in myocytes.
-
Immunohistochemical analysis of human placenta tissue labelling PGP with ab261736 shows moderate granular cytoplasmatic positivity in trophoblastic cells.
-
Immunofluorescent analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling PGP using ab261736 at 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.
-
All lanes : Anti-PGP antibody (ab261736) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T cell lysate
Lane 2 : PGP overexpression HEK-293T cell lysate
Predicted band size: 34 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab261736 has been referenced in 2 publications.
- Fu Q et al. Proteomics reveals MRPL4 as a high-risk factor and a potential diagnostic biomarker for prostate cancer. Proteomics 22:e2200081 (2022). PubMed: 36059095
- Li D et al. MicroRNA-375 represses tumor angiogenesis and reverses resistance to sorafenib in hepatocarcinoma. Cancer Gene Ther 28:126-140 (2021). PubMed: 32616906