Product nameAnti-PGP antibody
See all PGP primary antibodies
DescriptionRabbit polyclonal to PGP
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- WB: PGP overexpression HEK-293T cell lysate. IHC-P: Human liver tissue. ICC/IF: MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab261736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.4 µg/ml. Predicted molecular weight: 34 kDa.
Recombinant expression validation in WB using target protein overexpression.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
Tissue specificityDetected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level). The highest activities occur in skeletal muscle and cardiac muscle.
Sequence similaritiesBelongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.
- Information by UniProt
- PGP antibody
- PGP_HUMAN antibody
- PGPase antibody
- Phosphoglycolate phosphatase antibody
Immunofluorescent analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling PGP using ab261736 at 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.
Formalin/PFA-fixed paraffin-embedded human liver tissue labeling PGP using ab261736 at a 1/200 dilution.
Retrieval method: HIER pH6.
All lanes : Anti-PGP antibody (ab261736) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T cell lysate
Lane 2 : PGP overexpression HEK-293T cell lysate
Predicted band size: 34 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab261736 has not yet been referenced specifically in any publications.