Overview

  • Product name

  • Description

    Rabbit polyclonal to PGP9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human PGP9.
    Database link: NP_062571.1

  • Positive control

    • HeLa, 293T and mouse NIH3T3 whole cell lysates
  • General notes

     This product was previously labelled as ABCB9

     

Properties

Applications

Our Abpromise guarantee covers the use of ab86222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance

    The membrane associated protein encoded by this gene is a member of the superfamily of ATP binding cassette (ABC) transporters. ABC proteins transport various molecules across extra and intra cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half transporter has not yet been determined; however, this protein may play a role in lysosomes. Alternative splicing of this gene results in three known products which are likely to have different substrate specifications.
  • Cellular localization

    Integral membrane protein. Lysosomal. May be localized in the endoplasmic reticulum.
  • Database links

  • Alternative names

    • ABC transporter 9 protein antibody
    • ABCB 9 antibody
    • ATP binding cassette sub family B (MDR/TAP) member 9 antibody
    • ATP binding cassette sub family B member 9 precursor antibody
    • ATP binding cassette transporter 9 antibody
    • EST122234 antibody
    • hABCB9 antibody
    • KIAA1520 antibody
    • TAP like protein antibody
    • TAPL antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PGP9 with ab86222 at 1/200 (1µg/ml). Detection: DAB.

  • All lanes : Anti-PGP9 antibody (ab86222) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 84 kDa


    Exposure time: 10 seconds
  • Detection of human PGP9 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86222 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

    Detection: Chemiluminescence with an exposure time of 3 seconds.

References

This product has been referenced in:

  • Rodriguez-Navarro JA  et al. Inhibitory effect of dietary lipids on chaperone-mediated autophagy. Proc Natl Acad Sci U S A 109:E705-14 (2012). WB ; Mouse . Read more (PubMed: 22331875) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Question
Answer

Unfortunately, no we do not have the blocking peptide commercially available for this product. I apologize for the inconvenience.

Read More

Answer

Thank you for confirmation.

The antibody ab45371 is currently out of stock until 14th January. The items will be dispatched on 15th January with order number 1233746.

I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

Read More

Answer

Thank very much for your patience while laboratory was retesting the specificity of this antibody.

I can confirm that the antibody has passed the quality test again in reciprocal IP/WB so we have no doubt about the specificity of this product. I am not sure if you have tested the knockout with techniques other than PCR so it is difficult to understand why this antibody is binding to knockouts if the cell are absolute knockouts.

We unfortunately do not have any knockout lysates available so I am sorry the antibody cannot be further tested.

I hope this information is nevertheless helpful. Should you have any question please do not hesitate to contact me.

Read More

Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

Just to update you regarding further steps. Our laboratory has agreed for further testing of this antibody. The results are expected by the end of next week. I will contact you with more updates soon.

Thank you very much for your cooperation. I will look forward to hearing from you soon.

Read More

Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up