Anti-PGP9 antibody (ab86222)
Key features and details
- Rabbit polyclonal to PGP9
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PGP9 antibody -
Description
Rabbit polyclonal to PGP9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide corresponding to Human PGP9.
Database link: Q9NP78 -
Positive control
- HeLa, 293T and mouse NIH3T3 whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 84 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
The membrane associated protein encoded by this gene is a member of the superfamily of ATP binding cassette (ABC) transporters. ABC proteins transport various molecules across extra and intra cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. The function of this half transporter has not yet been determined; however, this protein may play a role in lysosomes. Alternative splicing of this gene results in three known products which are likely to have different substrate specifications. -
Cellular localization
Integral membrane protein. Lysosomal. May be localized in the endoplasmic reticulum. -
Database links
- Entrez Gene: 23457 Human
- Entrez Gene: 56325 Mouse
- Entrez Gene: 63886 Rat
- Omim: 605453 Human
- SwissProt: Q9NP78 Human
- SwissProt: Q9JJ59 Mouse
- SwissProt: Q9QYJ4 Rat
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Alternative names
- ABC transporter 9 protein antibody
- ABCB 9 antibody
- ATP binding cassette sub family B (MDR/TAP) member 9 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PGP9 with ab86222 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-PGP9 antibody (ab86222) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 10 seconds -
Detection of human PGP9 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86222 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab86222 has been referenced in 1 publication.
- Rodriguez-Navarro JA et al. Inhibitory effect of dietary lipids on chaperone-mediated autophagy. Proc Natl Acad Sci U S A 109:E705-14 (2012). WB ; Mouse . PubMed: 22331875