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  1. Link

    pgp95-antibody-13c4--i3c4-ab8189.pdf

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Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Parkin / PARK
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Validated using a knockout cell line

Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)

  • Datasheet
  • SDS
Reviews (34)Q&A (9)References (98)

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Immunocytochemistry - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)
  • Western blot - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)
  • Western blot - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)
  • Immunocytochemistry - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)

Key features and details

  • Mouse monoclonal [13C4 / I3C4] to PGP9.5
  • Suitable for: ICC, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-PGP9.5 antibody [13C4 / I3C4]
    See all PGP9.5 primary antibodies
  • Description

    Mouse monoclonal [13C4 / I3C4] to PGP9.5
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Guinea pig, Dog, Pig, Zebrafish
  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate. Human, mouse and rat brain tissue lysate. Rat cortex tissue lysate. SHSY-5Y whole cell lysate. Human spinal cord tissue lysate. IHC-P: Rat pancreas tissue. ICC: Primary rat neurons/glia, DIV14 cells.
  • General notes

    This antibody labels the neuronal cell bodies and axons in central and peripheral neural system. Small nerve fibers in peripheral tissues, neuroendocrine cells in normal pituitary thyroid, pancreas, and gastrointestinal tract, as well as derived tumors are also stained with this antibody.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Some batches contain L-Arginine or BSA as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Primary antibody notes

    This antibody labels the neuronal cell bodies and axons in central and peripheral neural system. Small nerve fibers in peripheral tissues, neuroendocrine cells in normal pituitary thyroid, pancreas, and gastrointestinal tract, as well as derived tumors are also stained with this antibody.
  • Clonality

    Monoclonal
  • Clone number

    13C4 / I3C4
  • Isotype

    IgG2a
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Parkin / PARK
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Deubiquitination
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Neuronal
    • Neuroscience
    • Diseases

Associated products

  • Alternative Versions

    • Anti-PGP9.5 antibody [13C4 / I3C4] - BSA and Azide free (ab180872)
    • HRP Anti-PGP9.5 antibody [13C4 / I3C4] (ab197735)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant human PGP9.5 protein (ab53962)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use a concentration of 5 µg/ml.
IHC-P (16)
Use a concentration of 0.5 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (7)
Use a concentration of 5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Notes
ICC
Use a concentration of 5 µg/ml.
IHC-P
Use a concentration of 0.5 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • Function

    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • Tissue specificity

    Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • Involvement in disease

    Parkinson disease 5
    Neurodegeneration with optic atrophy, childhood-onset
  • Sequence similarities

    Belongs to the peptidase C12 family.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Target information above from: UniProt accession P09936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7345 Human
    • Entrez Gene: 22223 Mouse
    • Entrez Gene: 396637 Pig
    • Entrez Gene: 29545 Rat
    • Entrez Gene: 101117250 Sheep
    • Omim: 191342 Human
    • SwissProt: P09936 Human
    • SwissProt: Q9R0P9 Mouse
    • SwissProt: Q6SEG5 Pig
    • SwissProt: Q00981 Rat
    • Unigene: 518731 Human
    • Unigene: 29807 Mouse
    • Unigene: 107213 Rat
    see all
  • Alternative names

    • Epididymis luminal protein 117 antibody
    • Epididymis secretory protein Li 53 antibody
    • HEL 117 antibody
    • HEL S 53 antibody
    • NDGOA antibody
    • Neuron cytoplasmic protein 9.5 antibody
    • OTTHUMP00000218137 antibody
    • OTTHUMP00000218139 antibody
    • OTTHUMP00000218140 antibody
    • OTTHUMP00000218141 antibody
    • Park 5 antibody
    • PARK5 antibody
    • PGP 9.5 antibody
    • PGP9.5 antibody
    • PGP95 antibody
    • Protein gene product 9.5 antibody
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin C terminal hydrolase antibody
    • Ubiquitin C terminal hydrolase L1 antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
    • Ubiquitin thioesterase L1 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L1 antibody
    • UCH-L1 antibody
    • UCHL1 antibody
    • UCHL1_HUMAN antibody
    see all

Images

  • Immunocytochemistry - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)
    Immunocytochemistry - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)

    ab8189 staining PGP9.5 in primary rat neurons/glia, DIV14 (prepared from E18 rat hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. SDHEP) cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab8189 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150121, Goat polyclonal Secondary Antibody to Mouse IgM - mu chain (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

    IHC-P image of PGP9.5 staining on P5 mouse skin sections using ab8189 (1/1000).

    The sections were de-paraffinized and subjected to heat meadiated antigen retrieval using citric acid. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab8189 was then incubated for 16 hours at 21°C. The secondary antibody used was Got polyclonal to anti-mouse IgG conjugated to biotin (1/200).

    See Abreview

  • Western blot - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)
    Western blot - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: PGP9.5 knockout HAP1 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab8189 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab8189 was shown to specifically react with PGP9.5 in wild-type HAP1 cells as signal was lost in PGP9.5 knockout cells. Wild-type and PGP9.5 knockout samples were subjected to SDS-PAGE. ab8189 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 5 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)
    Western blot - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)
    All lanes : Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189) at 5 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Rat Cortex Tissue Lysate
    Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 6 : Human spinal cord tissue lysate - total protein (ab29188)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa


    Exposure time: 1 minute


    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab8189 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
  • Immunocytochemistry - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)
    Immunocytochemistry - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)Image courtesy of QBMCellScience
    ab8189 (1/20) immunostaining neurons in mouse cortical primary cell culture.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

    IHC-P image of PGP9.5 staining on zebrafish brain using ab8189 (1/1000). The sections were subjected to heat mediated antigen retrieval using citric acid. The sections were then blocked using 1% BSA for 10 mins for 21°C. The primary antibody (ab8189) was incubated at a dilution of 1/1000 at 21°C for 16 hours. The secondary antibody used was undiluted goat polyclonal to Mouse IgG conjugated to biotin.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [13C4 / I3C4] (ab8189)

    IHC image of PGP9.5 staining in rat pancreas formalin-fixed, paraffin-embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8189, 0.02µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Protocols

  • Mouse on Mouse staining protocol

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (98)

Publishing research using ab8189? Please let us know so that we can cite the reference in this datasheet.

ab8189 has been referenced in 98 publications.

  • Gao R  et al. Macrophage-derived netrin-1 drives adrenergic nerve-associated lung fibrosis. J Clin Invest 131:N/A (2021). PubMed: 33393489
  • Yuan H  et al. Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine. Stem Cells Transl Med 10:922-938 (2021). PubMed: 33481357
  • Zhang Y  et al. Small extracellular vesicles ameliorate peripheral neuropathy and enhance chemotherapy of oxaliplatin on ovarian cancer. J Extracell Vesicles 10:e12073 (2021). PubMed: 33728031
  • Sadeghi L  et al. Proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy: potential targets in calcium regulatory network. Sci Rep 11:8252 (2021). PubMed: 33859251
  • de Melo GD  et al. COVID-19-related anosmia is associated with viral persistence and inflammation in human olfactory epithelium and brain infection in hamsters. Sci Transl Med 13:N/A (2021). PubMed: 33941622
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-10 of 43 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-PGP9.5 antibody [13C4 / I3C4]

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Lizard Cell (Uterus cryosections)
Permeabilization
No
Specification
Uterus cryosections
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Sam Dowland

Verified customer

Submitted Mar 03 2020

Immunohistochemistry (Frozen sections) abreview for Anti-PGP9.5 antibody [13C4 / I3C4]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (reconstructed epidermis + human sensitive neurones)
Permeabilization
Yes - PBS 0,5% tween
Specification
reconstructed epidermis + human sensitive neurones
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 26 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [13C4 / I3C4]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Aortic artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Aortic artery
Blocking step
BSA as blocking agent for 21 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 1°C
Fixative
10% normal buffered formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 10 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [13C4 / I3C4]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Breast tumor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
10% normal buffered formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 10 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [13C4 / I3C4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Ileum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Ileum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
10% normal buffered formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 09 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [13C4 / I3C4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium citrate pH6
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 09 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [13C4 / I3C4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Stomach)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
Stomach
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
10% normal buffered formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 09 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-PGP9.5 antibody [13C4 / I3C4]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Ferret Cell (Pancreas)
Permeabilization
Yes - Triton X-100
Specification
Pancreas
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

gulnara novbatova

Verified customer

Submitted Jul 27 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [13C4 / I3C4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
Yes - Triton X-100
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
HistoChoice
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 08 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [13C4 / I3C4]

Average
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 24 2016

1-10 of 43 Abreviews or Q&A

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