• Product name
    Anti-PGP9.5 antibody [31A3]
    See all PGP9.5 primary antibodies
  • Description
    Mouse monoclonal [31A3] to PGP9.5
  • Host species
  • Specificity
    Ab20559 stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibers in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumors of the diffuse neuroendocrine system.
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Native PGP 9.5 protein from brain

  • General notes

    This antibody clone is manufactured by Abcam.

    This monoclonal antibody to PGP9.5 has been knockout validated in Western blot. The expected band for PGP9.5 was observed in wild type cells and the band was not seen in knockout cells.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.



Our Abpromise guarantee covers the use of ab20559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


IHC-P Use at an assay dependent concentration.
ELISA 1/6000 - 1/25000.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Dilution optimised using Chromogenic detection.


  • Function
    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • Tissue specificity
    Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • Involvement in disease
    Parkinson disease 5
    Neurodegeneration with optic atrophy, childhood-onset
  • Sequence similarities
    Belongs to the peptidase C12 family.
  • Post-translational
  • Cellular localization
    Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis luminal protein 117 antibody
    • Epididymis secretory protein Li 53 antibody
    • HEL 117 antibody
    • HEL S 53 antibody
    • NDGOA antibody
    • Neuron cytoplasmic protein 9.5 antibody
    • OTTHUMP00000218137 antibody
    • OTTHUMP00000218139 antibody
    • OTTHUMP00000218140 antibody
    • OTTHUMP00000218141 antibody
    • Park 5 antibody
    • PARK5 antibody
    • PGP 9.5 antibody
    • PGP9.5 antibody
    • PGP95 antibody
    • Protein gene product 9.5 antibody
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin C terminal hydrolase antibody
    • Ubiquitin C terminal hydrolase L1 antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
    • Ubiquitin thioesterase L1 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L1 antibody
    • UCH-L1 antibody
    • UCHL1 antibody
    • UCHL1_HUMAN antibody
    see all


  • Immunohistochemical analysis of rat emybronic rectal tissue, staining PGP9.5 with ab20559.

    After antigen retrieval, the sections were treated and incubated with primary antibody (1/200), followed by an HRP-conjugated donkey anti-mouse IgG (1/200). Staining was detected using DAB.
  • Lane 1: Wild type HAP1 whole cell lysate (20 µg)              
    Lane 2: UCHL1 (KO) knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: SH-SY5Y whole cell lysate (20 µg)
    Lane 4: Hek293 whole cell lysate (20 µg)              

    Lanes 1 - 4: Merged signal (red and green). Green - ab20559 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab20559 detected the expected band for PGP9.5 in wild type cells and the band was not seen in PGP9.5/UCHL1 knockout cells. Wild-type and UCHL1 knockout samples were subjected to SDS-PAGE.  Ab20559 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Overlay histogram showing SH-SH5Y cells stained with ab20559 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20559, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-PGP9.5 antibody [31A3] (ab20559) at 5 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Brain (Mouse) Tissue Lysate
    Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 4 minutes


This product has been referenced in:
  • Mtango NR  et al. Essential role of maternal UCHL1 and UCHL3 in fertilization and preimplantation embryo development. J Cell Physiol 227:1592-603 (2012). ICC/IF ; Mouse . Read more (PubMed: 21678411) »
  • Saygili E  et al. Age-related regional differences in cardiac nerve growth factor expression. Age (Dordr) 34:659-67 (2012). WB ; Rat . Read more (PubMed: 21559866) »
See all 8 Publications for this product

Customer reviews and Q&As


Thank you for contacting us. We have two anti-PGP9.5 antibodies in our catalogue which have been tested using ELISA which I can suggest to you. ab20559 is a mouse monoclonal, reactive against human and rat PGP9.5 and ab53057 is a rabbit polyclonal reactive against human  PGP9.5 (and is predicted to react with rat protein). Whilst these have been used in ELISAs before, they have not to our knowledge been used as a pair for a sandwich ELISA. Unfortunately we do not have any information for either antibody about the affinity or the "on-rate". I am sorry that I could not be of more help. If you require any further information please do not hesitate to ask.

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