- Datasheet
- Specific References (21)
- Protocols
Overview
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Product nameAnti-PGP9.5 antibody
See all PGP9.5 primary antibodies -
DescriptionRabbit polyclonal to PGP9.5
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Fr, WB, IHC-P, IHC (PFA fixed), ICC, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human, Pig
Predicted to work with: Horse
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Immunogen
Synthetic peptide:
ASSEDTLLKDAAKVCR
, corresponding to amino acids 176 - 191 of Human PGP9.5. -
Positive control
- Human brain lysate.
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General notesSerum may become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: Whole serum -
PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab10404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/2000. | |
| WB | 1/1000. Predicted molecular weight: 26.8 kDa.Can be blocked with Human PGP9.5 peptide (ab38203). | |
| IHC-P | 1/100 - 1/300. | |
| IHC (PFA fixed) | 1/200. PubMed: 17508253 | |
| ICC | Use at an assay dependent concentration. | |
| ICC/IF | 1/1000. |
Target
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FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
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Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
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Involvement in diseaseParkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
Sequence similaritiesBelongs to the peptidase C12 family.
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Post-translational
modificationsO-glycosylated. -
Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
- Information by UniProt
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Database links
- Entrez Gene: 100033838 Horse
- Entrez Gene: 7345 Human
- Entrez Gene: 22223 Mouse
- Entrez Gene: 396637 Pig
- Entrez Gene: 29545 Rat
- Omim: 191342 Human
- SwissProt: Q9GM50 Horse
- SwissProt: P09936 Human
see all -
Alternative names
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody (ab10404)Image from McIver SC et al., PLoS One. 2012;7(4):e35553. Epub 2012 Apr 20. Fig 1.; doi:10.1371/journal.pone.0035553; April 20, 2012, PLoS ONE 7(4): e35553.Immunofluorescence analysis of mouse gonocytes, staining PGP9.5 with ab10404.
Cells were permabilized with 0.025% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Cells were incubated with primary antibody (1/20) overnight at 4°C. An AlexaFluor®594-conjugated anti-rabbit IgG was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody (ab10404)This image is courtesy of an anonymous Abreviewab10404 at a 1/500 dilution staining human cancer prostate tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections).Following heat mediated antigen retrieval the tissue was fixed with formaldehyde and blocked with 20% serum. The tissue was incubated with the primary antibody for 1 hour and then with an HRP conjugated rabbit polyclonal antibody. -
Western blot - Anti-PGP9.5 antibody (ab10404)Anti-PGP9.5 antibody (ab10404) at 1/1000 dilution + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 26.8 kDa
Observed band size: 27 kDa (why is the actual band size different from the predicted?)
Additional bands at: 48 kDa, 70 kDa, 73 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Frozen sections) - Anti-PGP9.5 antibody (ab10404)ab10404 at a dilution of 1/1000, detecting PGP9.5 in cortical neurons (Alexa 488 secondary at 1/2000) on rat brain tissue sections prepared for free floating IHC (4% PFA fixed, 30
µ m thick coronal sections; see protocol link for detailed description). Image taken with a 20x objective. No labeling observed following omission of primary antibody.Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody (ab10404)Image is courtesy of Dr Brett McKinnonab10404 staining PGP9.5 in Mouse Endometriotic tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in 3% BSA) for 20 hours at 4°C. An undiluted Biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunohistochemistry (Frozen sections) - Anti-PGP9.5 antibody (ab10404)ab10404 at a dilution of 1/2000, staining PGP9.5 in rat dorsal root ganglion by Immunohistochemistry (PFA perfusion fixed frozen sections).
Protocols
References
This product has been referenced in:
- Dias DO et al. Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord Injury. Cell 173:153-165.e22 (2018). Read more (PubMed: 29502968) »
- Chan KY et al. Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems. Nat Neurosci 20:1172-1179 (2017). IHC ; Mouse . Read more (PubMed: 28671695) »
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