Key features and details
- Rabbit polyclonal to PGP9.5
- Suitable for: IHC-Fr, WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Product nameAnti-PGP9.5 antibody
See all PGP9.5 primary antibodies
DescriptionRabbit polyclonal to PGP9.5
Tested applicationsSuitable for: IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Rat, Human, Pig
Predicted to work with: Horse
General notesSerum may become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab10404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 26.8 kDa.|
FunctionUbiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Tissue specificityFound in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
Involvement in diseaseParkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset
Sequence similaritiesBelongs to the peptidase C12 family.
Cellular localizationCytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
- Information by UniProt
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
Anti-PGP9.5 antibody (ab10404) at 1/1000 dilution + Human brain tissue lysate - total protein (ab29466) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 26.8 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa, 70 kDa, 73 kDa. We are unsure as to the identity of these extra bands.
ab10404 at a dilution of 1/1000, detecting PGP9.5 in cortical neurons (Alexa 488 secondary at 1/2000) on rat brain tissue sections prepared for free floating IHC (4% PFA fixed, 30
µm thick coronal sections; see protocol link for detailed description). Image taken with a 20x objective. No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
ab10404 at a dilution of 1/2000, staining PGP9.5 in rat dorsal root ganglion by Immunohistochemistry (PFA perfusion fixed frozen sections).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab10404 has been referenced in 32 publications.
- Goto K et al. Protective mechanism against age-associated changes in the peripheral nerves. Life Sci 253:117744 (2020). PubMed: 32371065
- Su W et al. Angiogenesis stimulated by elevated PDGF-BB in subchondral bone contributes to osteoarthritis development. JCI Insight 5:N/A (2020). PubMed: 32208385
- Cerles O et al. Preventive action of benztropine on platinum-induced peripheral neuropathies and tumor growth. Acta Neuropathol Commun 7:9 (2019). PubMed: 30657060
- García-Piqueras J et al. Ageing of the somatosensory system at the periphery: age-related changes in cutaneous mechanoreceptors. J Anat N/A:N/A (2019). PubMed: 30924930
- Lionarons JM et al. Dystrophin is expressed in smooth muscle and afferent nerve fibers in the rat urinary bladder. Muscle Nerve 60:202-210 (2019). PubMed: 31095755