Anti-PGP9.5 antibody (ab10404)

Thank you for your question. The protein PGP9.5 is expressed in neurons and most brain tissue, especially from the cerebral cortex, will be a good positive tissue control. A negative tissue control is more difficult to recommend but the  Human Protein Atlas has RNA and protein expression data for tissues at http://www.proteinatlas.org/ENSG00000154277-UCHL1/tissue, and cell line data at http://www.proteinatlas.org/ENSG00000154277-UCHL1/cell/HPA005993.

Thank you for contacting us.

According to UniGene, the RNA for this protein can be found quite abundantly in human pancreas. Therefore, it shouldn't be a problem to find a suitable antibody which has been tested in human and IHC-P.

We have two polyclonal antibodies, which have been used in various references and Abreviews. However, only

https://www.abcam.com/index.html?datasheet=15503 (or use the following: https://www.abcam.com/index.html?datasheet=15503).

has been tested by us so far in-house in pancreas tissue.

The other antibody whith a lot of refferences and Abreviews would be

https://www.abcam.com/index.html?datasheet=10404 (or use the following: https://www.abcam.com/index.html?datasheet=10404).

If they would prefer a monoclonal antibody, I would like to suggest

https://www.abcam.com/index.html?datasheet=8189 (or use the following: https://www.abcam.com/index.html?datasheet=8189).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

ab10404 is a whole serum product and the specific antibody concentration cannot be determined.

I am sorry for not being able to provide useful information this time. Please do not hesitate to contact us if you need any more advice or information in other aspects.

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https://www.abcam.com/abreviews

Thank you for taking the time to complete our questionnaire.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

Reviewing this case, I would like to offer some suggestions to help optimize the results from ab10404. I would also appreciate if you can confirm some further details:

1. I am sorry I am not able to trace any orders for this antibody from Chile.
Please confirm the order number and date of purchase and details of where you purchased from. If you have ordered through a distributor, they will have the order number reference from us.

2. I can recommend to try different time points for antigen retrieval as this can sometimes help optimize the results. For example, try 3, 5, 10 and 20 minutes.

3. I can recommend to continue to use a lower concentration of the antibody, 1:10000 or1:20000. However, I suggest to try adding 0.2% Tween to the antibody dilution buffers and the wash buffers. This will help to solubilise the antibody and also help to wash away any excess antibody.

4. We recommend not to mix blocking agents in one experiment as it can affect the results. I can suggestincreasing the amount of blocking agent in the blocking buffer, try 5% or 10% serum only. And alsoadd 2% Serum to the antibody dilution buffer.

5. Please confirm if the secondary antibody is working well with other rabbit primary antibodies? I can suggest it would be important to consider including a no primary control to assess if the secondary antibody is binding non specifically. What were the results from the no primary control? The concentration of the secondary may need to be reduced to help optimize the results.

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details including the results of the no primary control.

TThank you for calling Abcam regarding positive controls.

AB10404 has been used in IHC-P on lung sections in the following publication:Liu Yet al.Human metapneumovirus establishes persistent infection in the lungs of mice and is reactivated by glucocorticoid treatment.J Virol83:6837-48 (2009).http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=19357175&dopt=Abstract.
This product has also been used by customers in human prostate carcinoma tissue to good effect. More information about this can be seen at this link:https://www.abcam.com/index.html?datasheet=10404&tab=abreviews&intabreviewid=5255

In regards to AB8874: The publication,Liu Set al.Cross-talk between Schwann cells and neuroblasts influences the biology of neuroblastoma xenografts.Am J Pathol166:891-900 (2005).http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15743800&dopt=Abstract,uses human schwannoma as a positive control for anti-human p75NGFRantibody.

I hope that this information is helpful. Please let me know if you have any questions.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

I would like to reassure you that this antibody is tested and covered by our guarantee forIHC-P. I am sorry Ido not know the species you are testing but it is guaranteed for Mouse, Rat, HumanandPig. Before deciding how to proceed, I would like to investigate this particular case further for you, and also obtain some further information for our quality records. In order to do this, I have enclosed a questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

We will happily offer technical support and in the event that a product is not functioning in the applications and speciescited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.

Order Details
Antibody code:

Lot number:

Purchase order number
or preferably Abcam order number:


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)


Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)


Optimization attempts (problem solving)
How many times have you tried the IHC?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide and image which woudl help us to assess the results