Overview

  • Product name

  • Description

    Rabbit polyclonal to PGP9.5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant fragment within Human PGP9.5 (internal sequence). The exact sequence is proprietary.
    Database link: P09936

  • Positive control

    • WB: Rat brain extract; A549 and H1299 whole cell lysates; Mouse brain lysate. IHC-Fr: E13.5 rat brain tissue. IHC-P: Human oral cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/1000.
IHC-Fr 1/100 - 1/1000.

Target

  • Function

    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • Tissue specificity

    Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • Involvement in disease

    Parkinson disease 5
    Neurodegeneration with optic atrophy, childhood-onset
  • Sequence similarities

    Belongs to the peptidase C12 family.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Epididymis luminal protein 117 antibody
    • Epididymis secretory protein Li 53 antibody
    • HEL 117 antibody
    • HEL S 53 antibody
    • NDGOA antibody
    • Neuron cytoplasmic protein 9.5 antibody
    • OTTHUMP00000218137 antibody
    • OTTHUMP00000218139 antibody
    • OTTHUMP00000218140 antibody
    • OTTHUMP00000218141 antibody
    • Park 5 antibody
    • PARK5 antibody
    • PGP 9.5 antibody
    • PGP9.5 antibody
    • PGP95 antibody
    • Protein gene product 9.5 antibody
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin C terminal hydrolase antibody
    • Ubiquitin C terminal hydrolase L1 antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
    • Ubiquitin thioesterase L1 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L1 antibody
    • UCH-L1 antibody
    • UCHL1 antibody
    • UCHL1_HUMAN antibody
    see all

Images

  • Paraffin-embedded human oral cancer tissue stained for PGP9.5 using ab227104 at 1/100 dilution in immunohistochemical analysis.

  • Anti-PGP9.5 antibody (ab227104) at 1/1000 dilution + Rat brain extract at 50 µg

    Predicted band size: 24 kDa



    12% SDS-PAGE gel.

  • Frozen sectioned E13.5 rat brain tissue stained for PGP9.5 (green) using ab227104 at 1/500 dilution in immunohistochemical analysis.

    Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody at 1/500 dilution.

    Blue: DAPI.

  • All lanes : Anti-PGP9.5 antibody (ab227104) at 1/5000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 24 kDa



    12% SDS-PAGE gel.

  • Anti-PGP9.5 antibody (ab227104) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 24 kDa



    12% SDS-PAGE gel.

References

ab227104 has not yet been referenced specifically in any publications.

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