Overview

  • Product name

  • Description

    Rabbit polyclonal to PGP9.5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PGP9.5 (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
    Database link: P09936

  • Positive control

    • WB: HEK-293T, A549 and H1299 whole cell extract; rat and mouse brain tissue extract. IHC-Fr: Mouse cerebellum tissue; rat E13.5 brain tissue. IHC-P: SEROUS OVCA xenograft tissue. ICC/IF: DIV9 rat E18 primary cortical neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab227157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 24 kDa.
IHC-Fr 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • Tissue specificity

    Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • Involvement in disease

    Parkinson disease 5
    Neurodegeneration with optic atrophy, childhood-onset
  • Sequence similarities

    Belongs to the peptidase C12 family.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Epididymis luminal protein 117 antibody
    • Epididymis secretory protein Li 53 antibody
    • HEL 117 antibody
    • HEL S 53 antibody
    • NDGOA antibody
    • Neuron cytoplasmic protein 9.5 antibody
    • OTTHUMP00000218137 antibody
    • OTTHUMP00000218139 antibody
    • OTTHUMP00000218140 antibody
    • OTTHUMP00000218141 antibody
    • Park 5 antibody
    • PARK5 antibody
    • PGP 9.5 antibody
    • PGP9.5 antibody
    • PGP95 antibody
    • Protein gene product 9.5 antibody
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin C terminal hydrolase antibody
    • Ubiquitin C terminal hydrolase L1 antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
    • Ubiquitin thioesterase L1 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L1 antibody
    • UCH-L1 antibody
    • UCHL1 antibody
    • UCHL1_HUMAN antibody
    see all

Images

  • Paraffin-embedded SEROUS OVCA (human) xenograft tissue stained for PGP9.5 with ab227157 at 1/300 dilution in immunohistochemical analysis.

  • Frozen section of E13.5 rat brain tissue stained for PGP9.5 using ab227157 at 1/250 dilution in immunohistochemical analysis (green)

    The nuclear counter stain is fluoroshield with DAPI (blue). Beta-Tubulin 3 is detected with an anti-beta-Tubulin 3 antibody (red).

  • All lanes : Anti-PGP9.5 antibody (ab227157) at 1/3000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T treated with PGP9.5 shRNA, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 24 kDa



    12% SDS-PAGE

  • 4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for PGP9.5 (green) using ab227157 at 1/500 dilution in ICC/IF.

    The nuclear counter stain is Fluoroshield with DAPI. Beta-Tubulin is detected with an anti-beta-Tubulin antibody (red).

  • Anti-PGP9.5 antibody (ab227157) at 1/10000 dilution + Rat brain tissue extract at 50 µg

    Developed using the ECL technique.

    Predicted band size: 24 kDa



    12% SDS-PAGE

  • Frozen section of adult mouse cerebellum tissue stained for PGP9.5 using ab227157 at 1/250 dilution in immunohistochemical analysis (green)

    The nuclear counter stain is fluoroshield with DAPI (blue). Beta-Tubulin is detected with an anti-beta-Tubulin antibody (red).

  • All lanes : Anti-PGP9.5 antibody (ab227157) at 1/5000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 24 kDa



    12% SDS PAGE

  • Anti-PGP9.5 antibody (ab227157) at 1/5000 dilution + Mouse brain tissue extract at 50 µg

    Developed using the ECL technique.

    Predicted band size: 24 kDa



    12 % SDS-PAGE

References

ab227157 has not yet been referenced specifically in any publications.

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